ArticleNature protocols2012
Quantification of adenosine-to-inosine editing of microRNAs using a conventional method.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Inosine-Induced Base Pairing Diversity during Reverse Transcription.ACS chemical biology · 2024Article
- The transcribed ultraconserved region uc.160+ enhances processing and A-to-I editing of the miR-376 cluster: hypermethylation improves glioma prognosis.Molecular oncology · 2022Article
- Review
- Identification of miRNAs Contributing to the Broad-Spectrum and Durable Blast Resistance in the Yunnan Local Rice Germplasm.Frontiers in plant science · 2021Article
- The MicroRNA Family Gets Wider: The IsomiRs Classification and Role.Frontiers in cell and developmental biology · 2021Review
- Identification and rescue of a tRNA wobble inosine deficiency causing intellectual disability disorder.RNA (New York, N.Y.) · 2020Article
- Antagonism between splicing and microprocessor complex dictates the serum-induced processing of lnc-RNA (New York, N.Y.) · 2020Article
- Homoplasmic mitochondrial tRNANeurology. Genetics · 2020Article
- Role of phasiRNAs from two distinct phasing frames of GhMYB2 loci in cis- gene regulation in the cotton genome.BMC plant biology · 2020Article
- Article
- Formation of tRNA Wobble Inosine in Humans Is Disrupted by a Millennia-Old Mutation Causing Intellectual Disability.Molecular and cellular biology · 2019Article
- Altered editing level of microRNAs is a potential biomarker in lung adenocarcinoma.Cancer science · 2018Article
- microRNA editing in seed region aligns with cellular changes in hypoxic conditions.Nucleic acids research · 2016Article
- Transcriptome-wide identification of adenosine-to-inosine editing using the ICE-seq method.Nature protocols · 2015Article
- A-to-I RNA Editing: Current Knowledge Sources and Computational Approaches with Special Emphasis on Non-Coding RNA Molecules.Frontiers in bioengineering and biotechnology · 2015Review
- Knowledge in the Investigation of A-to-I RNA Editing Signals.Frontiers in bioengineering and biotechnology · 2015Article
- VIRGO: visualization of A-to-I RNA editing sites in genomic sequences.BMC bioinformatics · 2013Article
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1 author.
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Abstract
In this protocol, I describe a method for measuring the frequency of adenosine-to-inosine RNA editing of primary, precursor and mature forms of specific microRNAs (miRNAs) derived from the same source. The procedure involves reverse transcription (RT)-PCR amplification of regions containing the editing sites followed by subcloning of the PCR products and sequencing. In contrast to deep sequencing, this method does not require any specialized equipment. Pri-miRNAs, which are relatively long primary transcripts, are amplified using a conventional RT-PCR method. Therefore, this method can be adapted for any known RNA-editing sites. In contrast, 3' polyadenylation followed by 5' adaptor ligation is indispensable for amplification of pre-miRNAs and mature miRNAs. The complete protocol takes ∼1 week. I also include details of direct sequence analysis of the PCR products derived from pri-miRNAs as an alternative method. Although it is not as precise as the subcloning method, this procedure enables us to study RNA-editing events of many samples.
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