ArticleJournal of neuroscience methods2012
FluoroMyelin™ Red is a bright, photostable and non-toxic fluorescent stain for live imaging of myelin.
Article in Journal of neuroscience methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Quantitative Comparison of Multi-Echo Spin Echo and Multi-Echo Gradient Echo Myelin Water Imaging in a Panel of Mbp Enhancer-Edited Mouse Lines.Magnetic resonance in medicine · 2026Article
- Investigation of the myelin-amyloid interplay in Alzheimer's disease: insights from novel dsCMA imaging in mouse and human brains.Acta neuropathologica communications · 2026Article
- Advances in multiscale myelin imaging: from classical histology to functional insights.Frontiers in cellular neuroscience · 2026Review
- Microglia-derived APOE2 improves remyelination even in the presence of endogenous APOE4.Journal of neuroinflammation · 2025Article
- Myelinated fiber labeling and orientation mapping of the human brain with light-sheet fluorescence microscopy.NeuroImage · 2025Article
- Trigeminal root demyelination is sufficient to induce trigeminal neuralgia-like facial pain in adult rodents.The journal of headache and pain · 2025Article
- Birefringence microscopy enables rapid, label-free quantification of myelin debris following induced cortical injury.Neurophotonics · 2025Article
- Evaluating the Protective Effects of Silymarin Along with a Cholesterol-Supplemented Diet Against Demyelination in an EAE Mouse Model of Multiple Sclerosis: Lipidomic and Inflammatory Aspects.Molecular neurobiology · 2025Article
- Label-free and fluorescence imaging of oligodendrocytes and myelin.Npj imaging · 2025Review
- Key genes and processes affected by atorvastatin treatment in mouse diaphragm muscle.Archives of toxicology · 2025Article
- Sex-chromosome complement and Activin-A shape the therapeutic potential of TNFR2 activation in a model of MS and CNP.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Fluorescent probes in autoimmune disease research: current status and future prospects.Journal of translational medicine · 2025Review
- Multiplex Immunofluorescent Batch Labeling of Marmoset Brain Sections.Brain and behavior · 2025Article
- A molecularly defined subpopulation of oligodendrocyte precursor cells controls the generation of myelinating oligodendrocytes during postnatal development.PLoS biology · 2024Article
- NF-κB subunits RelA and c-Rel selectively control CD4+ T cell function in multiple sclerosis and cancer.The Journal of experimental medicine · 2024Article
- Practical considerations for birefringence microscopy of myelin structure: Microscope design and tissue processing for effective imaging.Imaging neuroscience (Cambridge, Mass.) · 2024Article
- Revealing the three-dimensional murine brain microstructure by contrast-enhanced computed tomography.Frontiers in neuroscience · 2023Article
- A near-infrared AIE fluorescent probe for myelin imaging: From sciatic nerve to the optically cleared brain tissue in 3D.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Inducible knockout of Clec16a in mice results in sensory neurodegeneration.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
FluoroMyelin™ Red is a commercially available water-soluble fluorescent dye that has selectivity for myelin. This dye is marketed for the visualization of myelin in brain cryosections, though it is also used widely to stain myelin in chemically fixed tissue. Here we have investigated the suitability of FluoroMyelin™ Red as a vital stain for live imaging of myelin in myelinating co-cultures of Schwann cells and dorsal root ganglion neurons. We show that addition of FluoroMyelin™ Red to the culture medium results in selective staining of myelin sheaths, with an optimal staining time of 2h, and has no apparent adverse effect on the neurons, their axons, or the myelinating cells at the light microscopic level. The fluorescence is bright and photostable, permitting long-term time-lapse imaging. After rinsing the cultures with medium lacking FluoroMyelin™ Red, the dye diffuses out of the myelin with a half life of about 130 min resulting in negligible fluorescence remaining after 18-24h. In addition, the large Stokes shift exhibited by FluoroMyelin™ Red makes it possible to readily distinguish it from popular and widely used green and red fluorescent probes such as GFP and mCherry. Thus FluoroMyelin™ Red is a useful reagent for live fluorescence imaging studies on myelinated axons.
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