ArticleJournal of proteome research2012
High density diffusion-free nanowell arrays.
Article in Journal of proteome research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Serological profiling of Crohn's disease and ulcerative colitis patients reveals anti-microbial antibody signatures.World journal of gastroenterology · 2022Trial
- Anti-Epstein-Barr virus (EBV) antibodies in EBV-associated gastric carcinoma.Infectious agents and cancer · 2026Article
- Unbiased autoantibody screening using nucleic acid protein programmable array in pediatric autoimmune neuropsychiatric disorder associated with streptococcal infections.Frontiers in behavioral neuroscience · 2026Article
- Multiplexed proteomic biosensor platform for label-free real-time simultaneous kinetic screening of thousands of protein interactions.Communications biology · 2025Article
- Serum profiling of the antibody response to HPV in women with or without abnormal cervical cytology undergoing cervical cancer screening.Frontiers in immunology · 2025Article
- Comparative Analysis of Antimicrobial Antibodies between Mild and Severe COVID-19.Microbiology spectrum · 2023Article
- Modeling the sequence dependence of differential antibody binding in the immune response to infectious disease.PLoS computational biology · 2023Article
- Comparative Microbiomics Analysis of Antimicrobial Antibody Response between Patients with Lung Cancer and Control Subjects with Benign Pulmonary Nodules.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2023Article
- Exploration of autoantibody responses in canine diabetes using protein arrays.Scientific reports · 2022Article
- Scaling DNA data storage with nanoscale electrode wells.Science advances · 2021Article
- Identification of anti-Epstein-Barr virus (EBV) antibody signature in EBV-associated gastric carcinoma.Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association · 2021Article
- Lithographic Processes for the Scalable Fabrication of Micro- and Nanostructures for Biochips and Biosensors.ACS sensors · 2021Review
- Micro-scale technologies propel biology and medicine.Biomicrofluidics · 2021Article
- Micro/Nano Electrode Array Sensors: Advances in Fabrication and Emerging Applications in Bioanalysis.Frontiers in chemistry · 2020Review
- Multi-platform Approach for Microbial Biomarker Identification UsingFrontiers in cellular and infection microbiology · 2019Article
- Multiplexed Nucleic Acid Programmable Protein Arrays.Theranostics · 2017Article
- A Contra Capture Protein Array Platform for Studying Post-translationally Modified (PTM) Auto-antigenomes.Molecular & cellular proteomics : MCP · 2016Article
- Review
- Immunoproteomic Profiling of Antiviral Antibodies in New-Onset Type 1 Diabetes Using Protein Arrays.Diabetes · 2016Article
- Antiviral antibody profiling by high-density protein arrays.Proteomics · 2015Article
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
Proteomics aspires to elucidate the functions of all proteins. Protein microarrays provide an important step by enabling high-throughput studies of displayed proteins. However, many functional assays of proteins include untethered intermediates or products, which could frustrate the use of planar arrays at very high densities because of diffusion to neighboring features. The nucleic acid programmable protein array (NAPPA) is a robust in situ synthesis method for producing functional proteins just-in-time, which includes steps with diffusible intermediates. We determined that diffusion of expressed proteins led to cross-binding at neighboring spots at very high densities with reduced interspot spacing. To address this limitation, we have developed an innovative platform using photolithographically etched discrete silicon nanowells and used NAPPA as a test case. This arrested protein diffusion and cross-binding. We present confined high density protein expression and display, as well as functional protein-protein interactions, in 8000 nanowell arrays. This is the highest density of individual proteins in nanovessels demonstrated on a single slide. We further present proof of principle results on ultrahigh density protein arrays capable of up to 24000 nanowells on a single slide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.