ArticleMolecular microbiology2012
Minimal requirements for inhibition of MraY by lysis protein E from bacteriophage ΦX174.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Functional Activity of the Lysis Protein E From Phage ID52: Dependence on SecB for Efficient Host Cell Lysis.Biotechnology journal · 2026Article
- Phage lysis protein LysScience advances · 2025Article
- The mechanism of the phage-encoded protein antibiotic from ΦX174.Science (New York, N.Y.) · 2023Article
- Article
- Breaking down the cell wall: Still an attractive antibacterial strategy.Frontiers in microbiology · 2022Review
- Article
- Single-gene lysis in the metagenomic era.Current opinion in microbiology · 2020Review
- Phage single-gene lysis: Finding the weak spot in the bacterial cell wall.The Journal of biological chemistry · 2019Review
- T7 RNA polymerase-driven inducible cell lysis for DNA transfer from Escherichia coli to Bacillus subtilis.Microbial biotechnology · 2017Article
- Mutational analysis of the MS2 lysis protein L.Microbiology (Reading, England) · 2017Article
- Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli.Microbial cell factories · 2017Article
- The Membrane Steps of Bacterial Cell Wall Synthesis as Antibiotic Targets.Antibiotics (Basel, Switzerland) · 2016Review
- New Insight into the Catalytic Mechanism of Bacterial MraY from Enzyme Kinetics and Docking Studies.The Journal of biological chemistry · 2016Article
- Structural Insights into Protein-Protein Interactions Involved in Bacterial Cell Wall Biogenesis.Antibiotics (Basel, Switzerland) · 2016Review
- Genetically Determined Variation in Lysis Time Variance in the Bacteriophage φX174.G3 (Bethesda, Md.) · 2016Article
- Combining Genes from Multiple Phages for Improved Cell Lysis and DNA Transfer from Escherichia coli to Bacillus subtilis.PloS one · 2016Article
- Chemoenzymatic syntheses of water-soluble lipid I fluorescent probes.Tetrahedron letters · 2015Article
- Bacteriophage therapy against Enterobacteriaceae.Virologica Sinica · 2015Review
- Autotransporter-based antigen display in bacterial ghosts.Applied and environmental microbiology · 2015Article
- Delayed lysis confers resistance to the nucleoside analogue 5-fluorouracil and alleviates mutation accumulation in the single-stranded DNA bacteriophage ϕX174.Journal of virology · 2014Article
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Authors and funding
2 authors.
Funding
Abstract
The DNA phage ΦX174 encodes the integral membrane protein E whose expression leads to host cell lysis by inhibition of the peptidoglycan synthesis enzyme MraY. Here we use mutagenesis to characterize the molecular details of the E lysis mechanism. We find that a minimal 18-residue region with the modified wild-type sequences of the conserved transmembrane helix of E is sufficient to lyse host cells and that specific residues within and at the boundaries of this helix are important for activity. This suggests that positioning of the helix in the membrane is critical for interactions with MraY. We further characterize the interaction site of the transmembrane helix with MraY demonstrating E forms a stable complex with MraY. Triggering cell lysis by peptidoglycan synthesis inhibition is a traditional route for antimicrobial strategies. Understanding the mechanism of bacterial cell lysis by E will provide insights into new antimicrobial strategies using re-engineered E peptides.
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