ArticleBMC neuroscience2012
Infrared optical imaging of matrix metalloproteinases (MMPs) up regulation following ischemia reperfusion is ameliorated by hypothermia.
Article in BMC neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Optimizing intraluminal monofilament model of ischemic stroke in middle-aged Sprague-Dawley rats.BMC neuroscience · 2022Article
- Optical Imaging of Matrix Metalloproteinases Activity in Prostate Tumors in Mice.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Optical Detection of Distal Lung Enzyme Activity in Human Inflammatory Lung Disease.BME frontiers · 2021Article
- Duration of postoperative immobilization affects MMP activity at the healing graft-bone interface: Evaluation in a mouse ACL reconstruction model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Article
- Noninvasive detection of acute cerebral hypoxia and subsequent matrix-metalloproteinase activity in a mouse model of cerebral ischemia using multispectral-optoacoustic-tomography.Neurophotonics · 2018Article
- Review
- Non-invasive near-infrared fluorescence imaging of the neutrophil response in a mouse model of transient cerebral ischaemia.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2017Article
- Non-Invasive Monitoring of CNS MHC-I Molecules in Ischemic Stroke Mice.Theranostics · 2017Article
- Article
- Role of P38 MAPK on MMP Activity in Photothrombotic Stroke Mice as Measured using an Ultrafast MMP Activatable Probe.Scientific reports · 2015Article
- Multimodal imaging reveals temporal and spatial microglia and matrix metalloproteinase activity after experimental stroke.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2015Article
- Exosomes from Human Dental Pulp Stem Cells Suppress Carrageenan-Induced Acute Inflammation in Mice.Inflammation · 2015Article
- In-vitro and in-vivo imaging of MMP activity in cartilage and joint injury.Biochemical and biophysical research communications · 2015Article
- In vivo fluorescence reflectance imaging of protease activity in a mouse model of post-traumatic osteoarthritis.Osteoarthritis and cartilage · 2014Article
- Intraoperative imaging-guided cancer surgery: from current fluorescence molecular imaging methods to future multi-modality imaging technology.Theranostics · 2014Review
- Enzyme-activatable imaging probe reveals enhanced neutrophil elastase activity in tumors following photodynamic therapy.Journal of biomedical optics · 2013Article
- Magnetic resonance imaging of ischemia viability thresholds and the neurovascular unit.Sensors (Basel, Switzerland) · 2013Review
- Ex vivo effect of gold nanoparticles on porcine synovial membrane.Tissue barriers · 2013Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe investigated the use of a new MMP activatable probe MMPSense™ 750 FAST (MMPSense750) for in-vivo visualization of early MMP activity in ischemic stroke. Following middle cerebral artery occlusion (MCAO) optical imaging was performed. Near-infrared (NIR) fluorescent images of MMPSense activation were acquired using an Olympus fluorescent microscope, 1.25 x objective, a CCD camera and an appropriate filter cube for detecting the activated probe with peak excitation and emission at 749 and 775 nm, respectively. Images were acquired starting at 2 or 24 hours after reperfusion over the ipsilateral and contralateral cortex before and for 3 hours after, MMPSense750 was injected.
resultsIncreased intensities ipsilaterally were observed following MMPSense750 injection with ischemic injury but not in sham animals. There were significant ipsilateral and contralateral differences at 15 minutes (P <0.05) in early ischemic reperfusion and at time 0 in 24 hours post ischemia (P <0.05) which persisted at 180 minutes in both these groups (P <0.01), but not following sham surgery. The increase in ipsilateral signal intensity was attenuated by hypothermia. These observations corresponded with a significant increase in the total MMP-9 protein levels, 5 and 24 hours following ischemia reperfusion (P <0.05) and their reduction by hypothermia.
conclusionsMatrix-metalloproteinase upregulation in ischemia reperfusion can be imaged acutely in-vivo with NIRF using MMPSense750. Hypothermia attenuated both the optical increase in intensity after MMPSense750 and the increase in MMP-9 protein expression supporting the proof of concept that NIRF imaging using MMPSense can be used to assess potential therapeutic strategies for stroke treatment.
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