ArticlePlant methods2012
Robust reconstitution of active cell-cycle control complexes from co-expressed proteins in bacteria.
Article in Plant methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 50 citations in OpenAlex.
- Functional characterization of D-type cyclins involved in cell division in rice.BMC plant biology · 2024Article
- Sugar signals pedal the cell cycle!Frontiers in plant science · 2024Review
- A PSTAIRE-type cyclin-dependent kinase controls light responses in land plants.Science advances · 2022Article
- B1-type cyclins control microtubule organization during cell division in Arabidopsis.EMBO reports · 2022Article
- Plant CDKs-Driving the Cell Cycle through Climate Change.Plants (Basel, Switzerland) · 2021Review
- Bora phosphorylation substitutes in trans for T-loop phosphorylation in Aurora A to promote mitotic entry.Nature communications · 2021Article
- ThePlants (Basel, Switzerland) · 2021Article
- Defining the Cell Wall, Cell Cycle and Chromatin Landmarks in the Responses ofInternational journal of molecular sciences · 2021Article
- Ubiquitination in Plant Meiosis: Recent Advances and High Throughput Methods.Frontiers in plant science · 2021Review
- The CDK Inhibitor SIAMESE Targets Both CDKA;1 and CDKB1 Complexes to Establish Endoreplication in Trichomes.Plant physiology · 2020Article
- CDKD-dependent activation of CDKA;1 controls microtubule dynamics and cytokinesis during meiosis.The Journal of cell biology · 2020Article
- The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis.The EMBO journal · 2020Article
- A conserved but plant-specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The Cdk1/Cdk2 homolog CDKA;1 controls the recombination landscape inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- A multi-level response to DNA damage induced by aluminium.The Plant journal : for cell and molecular biology · 2019Article
- SWITCH 1/DYAD is a WINGS APART-LIKE antagonist that maintains sister chromatid cohesion in meiosis.Nature communications · 2019Article
- Functional Analysis of Short Linear Motifs in the Plant Cyclin-Dependent Kinase Inhibitor SIAMESE.Plant physiology · 2018Article
- Lineage- and stage-specific expressedDevelopment (Cambridge, England) · 2018Article
- Interregulation of CDKA/CDK1 and the Plant-Specific Cyclin-Dependent Kinase CDKB in Control of the Chlamydomonas Cell Cycle.The Plant cell · 2018Article
- Arabidopsis R1R2R3-Myb proteins are essential for inhibiting cell division in response to DNA damage.Nature communications · 2017Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCell proliferation is an important determinant of plant growth and development. In addition, modulation of cell-division rate is an important mechanism of plant plasticity and is key in adapting of plants to environmental conditions. One of the greatest challenges in understanding the cell cycle of flowering plants is the large families of CDKs and cyclins that have the potential to form many different complexes. However, it is largely unclear which complexes are active. In addition, there are many CDK- and cyclin-related proteins whose biological role is still unclear, i.e. whether they have indeed enzymatic activity. Thus, a biochemical characterization of these proteins is of key importance for the understanding of their function.
resultsHere we present a straightforward system to systematically express and purify active CDK-cyclin complexes from E. coli extracts. Our method relies on the concomitant production of a CDK activating kinase, which catalyzes the T-loop phosphorylation necessary for kinase activity. Taking the examples of the G1-phase cyclin CYCLIN D3;1 (CYCD3;1), the mitotic cyclin CYCLIN B1;2 (CYCB1;2) and the atypical meiotic cyclin SOLO DANCERS (SDS) in conjunction with A-, B1- and B2-type CDKs, we show that different CDKs can interact with various cyclins in vitro but only a few specific complexes have high levels of kinase activity.
conclusionsOur work shows that both the cyclin as well as the CDK partner contribute to substrate specificity in plants. These findings refine the interaction networks in cell-cycle control and pinpoint to particular complexes for modulating cell proliferation activity in breeding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.