ArticleBiomacromolecules2012
Thiol-ene photopolymerizations provide a facile method to encapsulate proteins and maintain their bioactivity.
Article in Biomacromolecules, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed.
- Body-responsive shape-memory polymers for biomedical applications.Bioactive materials · 2026Review
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- Versatility of Click Chemistry in Hydrogel Synthesis: From Molecular Strategies to Applications in Regenerative Medicine.Gels (Basel, Switzerland) · 2026Review
- A technique to create hydrogels with tethered concentration gradients of moleculesSoft matter · 2026Article
- Pulmonary Fibrosis-Focusing on the Future: Aspen Lung Conference 2024 Summary.American journal of respiratory cell and molecular biology · 2025Article
- De Novo Design of Integrin α5β1 Modulating Proteins to Enhance Biomaterial Properties.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Light-activated decellularized extracellular matrix-based bioinks for enhanced mechanical integrity.Materials today. Bio · 2025Review
- Chemical- and photo-activation of protein-protein thiol-ene coupling for protein profiling.Communications chemistry · 2025Article
- A Rheological Study on the Effect of Tethering Pro- and Anti-Inflammatory Cytokines into Hydrogels on Human Mesenchymal Stem Cell Migration, Degradation, and Morphology.Biomacromolecules · 2024Article
- Click Chemistry: Reaction Rates and Their Suitability for Biomedical Applications.Bioconjugate chemistry · 2024Review
- Spatial-Selective Volumetric 4D Printing and Single-Photon Grafting of Biomolecules within Centimeter-Scale Hydrogels via Tomographic Manufacturing.Advanced materials technologies · 2023Article
- Click chemistry functionalization of self-assembling peptide hydrogels.Journal of biomedical materials research. Part A · 2023Article
- Photocrosslinkable natural polymers in tissue engineering.Frontiers in bioengineering and biotechnology · 2023Review
- Towards spatially-organized organs-on-chip: Photopatterning cell-laden thiol-ene and methacryloyl hydrogels in a microfluidic device.Organs-on-a-chip · 2022Article
- Evaluation of a Novel Thiol-Norbornene-Functionalized Gelatin Hydrogel for Bioprinting of Mesenchymal Stem Cells.International journal of molecular sciences · 2022Article
- Hyaluronic acid hydrogels crosslinked via blue light-induced thiol-ene reaction for the treatment of rat corneal alkali burn.Regenerative therapy · 2022Article
- Optimized Photoclick (Bio)Resins for Fast Volumetric Bioprinting.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Single-Cell Microgels for Diagnostics and Therapeutics.Advanced functional materials · 2021Article
- Dual Functionalization of Gelatin for Orthogonal and Dynamic Hydrogel Cross-Linking.ACS biomaterials science & engineering · 2021Article
- Biofabrication Strategies for Musculoskeletal Disorders: Evolution towards Clinical Applications.Bioengineering (Basel, Switzerland) · 2021Review
12 more citing papers are in PubMed but not listed here.
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2 authors.
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Abstract
Photoinitiated polymerization remains a robust method for fabrication of hydrogels, as these reactions allow facile spatial and temporal control of gelation and high compatibility for encapsulation of cells and biologics. The chain-growth reaction of macromolecular monomers, such as acrylated PEG and hyaluronan, is commonly used to form hydrogels, but there is growing interest in step-growth photopolymerizations, such as the thiol-ene "click" reaction, as an alternative. Thiol-ene reactions are not susceptible to oxygen inhibition and rapidly form hydrogels using low initiator concentrations. In this work, we characterize the differences in recovery of bioactive proteins when exposed to similar photoinitiation conditions during thiol-ene versus acrylate polymerizations. Following exposure to chain polymerization of acrylates, lysozyme bioactivity was approximately 50%; after step-growth thiol-ene reaction, lysozyme retained nearly 100% of its prereaction activity. Bioactive protein recovery was enhanced 1000-fold in the presence of a thiol-ene reaction, relative to recovery from solutions containing identical primary radical concentrations, but without the thiol-ene components. When the cytokine TGFβ was encapsulated in PEG hydrogels formed via the thiol-ene reaction, full protein bioactivity was preserved.
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