ArticleBiomedical optics express2012
Light-induced Ca(2+) transients observed in widefield epi-fluorescence microscopy of excitable cells.
Article in Biomedical optics express, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- Live-cell STED microscopy enables 50 nm resolution imaging with preserved cell proliferation.Scientific reports · 2026Article
- Harnessing artificial intelligence to reduce phototoxicity in live imaging.Journal of cell science · 2024Article
- Between life and death: strategies to reduce phototoxicity in super-resolution microscopy.Journal of physics D: Applied physics · 2020Review
- A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric CaJournal of microscopy · 2018Article
- Biological effects and medical applications of infrared radiation.Journal of photochemistry and photobiology. B, Biology · 2017Review
- Widefield Two-Photon Excitation without Scanning: Live Cell Microscopy with High Time Resolution and Low Photo-Bleaching.PloS one · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the possibility that variations in the level of intracellular Ca(2+) in excitable cells might be induced as an artifact of the incoherent illumination that is being used to monitor transient responses. In order to avoid the fluctuations in power of an arc lamp source, a microscope using a light emitting diode that was calibrated accurately at low power levels, was constructed to provide good control over the dose of light applied to the biological specimen. We report here that higher powers of illumination increased the probability of occurrence of Ca(2+) transients even in the sub-mW range normally used to measure such transients in epi-fluorescence work, suggesting that caution should be exercised when designing experiments and interpreting data.
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