ArticleBiomedical optics express2012
Depth-resolved cellular microrheology using HiLo microscopy.
Article in Biomedical optics express, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed.
- Passive and Active Microrheology for Biomedical Systems.Frontiers in bioengineering and biotechnology · 2022Review
- Article
- Three-dimensional image cytometer based on widefield structured light microscopy and high-speed remote depth scanning.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
- Depth resolved hyperspectral imaging spectrometer based on structured light illumination and Fourier transform interferometry.Biomedical optics express · 2014Article
- Improving high resolution retinal image quality using speckle illumination HiLo imaging.Biomedical optics express · 2014Article
- Hyperglycemic and hyperlipidemic conditions alter cardiac cell biomechanical properties.Biophysical journal · 2014Article
- Characterization of partially lifted cell sheets.Tissue engineering. Part A · 2014Article
- Optically-sectioned two-shot structured illumination microscopy with Hilbert-Huang processing.Optics express · 2014Article
- Optical measurement of arterial mechanical properties: from atherosclerotic plaque initiation to rupture.Journal of biomedical optics · 2013Review
- Improvement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination.Biomedical optics express · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is increasingly important to measure cell mechanical properties in three-dimensional environments. Particle tracking microrheology (PTM) can measure cellular viscoelastic properties; however, out-of-plane data can introduce artifacts into these measurements. We developed a technique that employs HiLo microscopy to reduce out-of-plane contributions. This method eliminated signals from 90% of probes 0.5 μm or further from the focal plane, while retaining all in-plane probes. We used this technique to characterize live-cell bilayers and found that there were significant, frequency-dependent changes to the extracted cell moduli when compared to conventional analysis. Our results indicate that removal of out-of-plane information is vital for accurate assessments of cell mechanical properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.