ArticleFrontiers in plant science2012
A candidate gene-based association study of tocopherol content and composition in rapeseed (Brassica napus).
Article in Frontiers in plant science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 68 citations in OpenAlex.
- Using near-infrared reflectance spectroscopy (NIRS) and chemometrics for non-destructive estimation of the amount and composition of seed tocopherols inJournal of food science and technology · 2025Article
- A Summary of Two Decades of QTL and Candidate Genes That Control Seed Tocopherol Contents in Maize (Genes · 2024Review
- Transcriptional and protein structural characterization of homogentisate phytyltransferase genes in barley, wheat, and oat.BMC plant biology · 2023Article
- Genotypic variation of tocopherol content in a representative genetic population and genome-wide association study on tocopherol in rapeseed (Molecular breeding : new strategies in plant improvement · 2023Article
- Wheat and Rice beyond Phenolic Acids: Genetics, Identification Database, Antioxidant Properties, and Potential Health Effects.Plants (Basel, Switzerland) · 2022Review
- Deciphering the Genetic Inheritance of Tocopherols in Indian Mustard (Plants (Basel, Switzerland) · 2022Article
- Identification of Genomic Loci and Candidate Genes Related to Seed Tocopherol Content in Soybean.Plants (Basel, Switzerland) · 2022Article
- Multi-Functional Development and Utilization of Rapeseed: Comprehensive Analysis of the Nutritional Value of Rapeseed Sprouts.Foods (Basel, Switzerland) · 2022Article
- Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells.Chinese journal of integrative medicine · 2021Article
- Article
- Fatty Acid Composition, Phytochemistry, Antioxidant Activity on Seed Coat and Kernel ofFoods (Basel, Switzerland) · 2019Article
- Identification of quantitative trait loci for increased α-tocopherol biosynthesis in wild soybean using a high-density genetic map.BMC plant biology · 2019Article
- Deciphering the genetic basis for vitamin E accumulation in leaves and grains of different barley accessions.Scientific reports · 2019Article
- Validation of an updated Associative Transcriptomics platform for the polyploid crop species Brassica napus by dissection of the genetic architecture of erucic acid and tocopherol isoform variation in seeds.The Plant journal : for cell and molecular biology · 2018Article
- FromFrontiers in plant science · 2018Review
- Recent Advances in our Understanding of Tocopherol Biosynthesis in Plants: An Overview of Key Genes, Functions, and Breeding of Vitamin E Improved Crops.Antioxidants (Basel, Switzerland) · 2017Review
- Novel Loci Underlie Natural Variation in Vitamin E Levels in Maize Grain.The Plant cell · 2017Article
- Antioxidant (Tocopherol and Canolol) Content in Rapeseed Oil Obtained from Roasted Yellow-SeededJournal of the American Oil Chemists' Society · 2017Article
- Comparison of statistical models for nested association mapping in rapeseed (Brassica napus L.) through computer simulations.BMC plant biology · 2016Article
- Genetic Analysis of Reduced γ-Tocopherol Content in Ethiopian Mustard Seeds.TheScientificWorldJournal · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapeseed (Brassica napus L.) is the most important oil crop of temperate climates. Rapeseed oil contains tocopherols, also known as vitamin E, which is an indispensable nutrient for humans and animals due to its antioxidant and radical scavenging abilities. Moreover, tocopherols are also important for the oxidative stability of vegetable oils. Therefore, seed oil with increased tocopherol content or altered tocopherol composition is a target for breeding. We investigated the role of nucleotide variations within candidate genes from the tocopherol biosynthesis pathway. Field trials were carried out with 229 accessions from a worldwide B. napus collection which was divided into two panels of 96 and 133 accessions. Seed tocopherol content and composition were measured by HPLC. High heritabilities were found for both traits, ranging from 0.62 to 0.94. We identified polymorphisms by sequencing selected regions of the tocopherol genes from the 96 accession panel. Subsequently, we determined the population structure (Q) and relative kinship (K) as detected by genotyping with genome-wide distributed SSR markers. Association studies were performed using two models, the structure-based GLM + Q and the PK-mixed model. Between 26 and 12 polymorphisms within two genes (BnaX.VTE3.a, BnaA.PDS1.c) were significantly associated with tocopherol traits. The SNPs explained up to 16.93% of the genetic variance for tocopherol composition and up to 10.48% for total tocopherol content. Based on the sequence information we designed CAPS markers for genotyping the 133 accessions from the second panel. Significant associations with various tocopherol traits confirmed the results from the first experiment. We demonstrate that the polymorphisms within the tocopherol genes clearly impact tocopherol content and composition in B. napus seeds. We suggest that these nucleotide variations may be used as selectable markers for breeding rapeseed with enhanced tocopherol quality.
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