ArticleThe Journal of biological chemistry2012
A Salmonella typhimurium-translocated glycerophospholipid:cholesterol acyltransferase promotes virulence by binding to the RhoA protein switch regions.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Speaking the host language: howMicrobiology (Reading, England) · 2023Review
- Review
- The type three secretion system effector protein IpgB1 promotes Shigella flexneri cell-to-cell spread through double-membrane vacuole escape.PLoS pathogens · 2022Article
- Quantitative proteomic screen identifies annexin A2 as a host target for Salmonella pathogenicity island-2 effectors SopD2 and PipB2.Scientific reports · 2021Article
- ARHGEF26 enhances Salmonella invasion and inflammation in cells and mice.PLoS pathogens · 2021Article
- The Salmonella Secreted Effector SarA/SteE Mimics Cytokine Receptor Signaling to Activate STAT3.Cell host & microbe · 2020Article
- Article
- The Role of the Type III Secretion System in the Intracellular Lifestyle of Enteric Pathogens.Microbiology spectrum · 2019Review
- A systematic exploration of the interactions between bacterial effector proteins and host cell membranes.Nature communications · 2017Article
- Outer membrane vesicle-associated lipase FtlA enhances cellular invasion and virulence in Francisella tularensis LVS.Emerging microbes & infections · 2017Article
- What the SIF Is Happening-The Role of IntracellularFrontiers in cellular and infection microbiology · 2017Review
- Article
- Bacterial Sphingomyelinases and Phospholipases as Virulence Factors.Microbiology and molecular biology reviews : MMBR · 2016Review
- Targeting of the hydrophobic metabolome by pathogens.Traffic (Copenhagen, Denmark) · 2015Review
- Salmonellae interactions with host processes.Nature reviews. Microbiology · 2015Review
- Structural basis for the recruitment and activation of the Legionella phospholipase VipD by the host GTPase Rab5.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Bacterial pathogens commandeer Rab GTPases to establish intracellular niches.Traffic (Copenhagen, Denmark) · 2012Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Salmonella enterica serovar typhimurium translocates a glycerophospholipid:cholesterol acyltransferase (SseJ) into the host cytosol after its entry into mammalian cells. SseJ is recruited to the cytoplasmic face of the host cell phagosome membrane where it is activated upon binding the small GTPase, RhoA. SseJ is regulated similarly to cognate eukaryotic effectors, as only the GTP-bound form of RhoA family members stimulates enzymatic activity. Using NMR and biochemistry, this work demonstrates that SseJ competes effectively with Rhotekin, ROCK, and PKN1 in binding to a similar RhoA surface. The RhoA surface that binds SseJ includes the regulatory switch regions that control activation of mammalian effectors. These data were used to create RhoA mutants with altered SseJ binding and activation. This structure-function analysis supports a model in which SseJ activation occurs predominantly through binding to residues within switch region II. We further defined the nature of the interaction between SseJ and RhoA by constructing SseJ mutants in the RhoA binding surface. These data indicate that SseJ binding to RhoA is required for recruitment of SseJ to the endosomal network and for full Salmonella virulence for inbred susceptible mice, indicating that regulation of SseJ by small GTPases is an important virulence strategy of this bacterial pathogen. The dependence of a bacterial effector on regulation by a mammalian GTPase defines further how intimately host pathogen interactions have coevolved through similar and divergent evolutionary strategies.
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