ArticleNucleic acids research2012
Replicative resolution of integron cassette insertion.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Bacterial natural transformation drives cassette shuffling and simplifies recombination in chromosomal integrons.Nucleic acids research · 2025Article
- The recombination efficiency of the bacterial integron depends on the mechanical stability of the synaptic complex.Science advances · 2024Article
- Unbridled Integrons: A Matter of Host Factors.Cells · 2022Review
- Methods for the targeted sequencing and analysis of integrons and their gene cassettes from complex microbial communities.Microbial genomics · 2022Article
- Predicting the taxonomic and environmental sources of integron gene cassettes using structural and sequence homology of attC sites.Communications biology · 2021Article
- Cassette recruitment in the chromosomal Integron of Vibrio cholerae.Nucleic acids research · 2021Article
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- Structure-specific DNA recombination sites: Design, validation, and machine learning-based refinement.Science advances · 2020Article
- Polymorphisms of Gene Cassette Promoters of the Class 1 Integron in ClinicalFrontiers in microbiology · 2019Article
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- Characterization of Novel Integrons, InFrontiers in microbiology · 2017Article
- Characterization of the novel In1059 harbouring VIM gene cassette.Antimicrobial resistance and infection control · 2017Article
- Efficiency of integron cassette insertion in correct orientation is ensured by the interplay of the three unpaired features of attC recombination sites.Nucleic acids research · 2016Article
- The evolutionary dynamics of integrons in changing environments.The ISME journal · 2016Article
- Unmasking the ancestral activity of integron integrases reveals a smooth evolutionary transition during functional innovation.Nature communications · 2016Article
- Diversity of Class 1 Integron Gene Cassette Rearrangements Selected under Antibiotic Pressure.Journal of bacteriology · 2015Article
- Integrons: past, present, and future.Microbiology and molecular biology reviews : MMBR · 2014Review
- Bacterial genome instability.Microbiology and molecular biology reviews : MMBR · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Site-specific recombination catalyzed by tyrosine recombinases follows a common pathway consisting of two consecutive strand exchanges. The first strand exchange generates a Holliday junction (HJ), which is resolved by a second strand exchange. In integrons, attC sites recombine as folded single-stranded substrates. Only one of the two attC site strands, the bottom one, is efficiently bound and cleaved by the integrase during the insertion of gene cassettes at the double-stranded attI site. Due to the asymmetry of this complex, a second strand exchange on the attC bottom strand (bs) would form linearized abortive recombination products. We had proposed that HJ resolution would rely on an uncharacterized mechanism, probably replication. Using an attC site carried on a plasmid with each strand specifically tagged, we followed the destiny of each strand after recombination. We demonstrated that only one strand, the one carrying the attC bs, is exchanged. Furthermore, we show that the recombination products contain the attC site bs and its entire de novo synthesized complementary strand. Therefore, we demonstrate the replicative resolution of single-strand recombination in integrons and rule out the involvement of a second strand exchange of any kind in the attC×attI reaction.
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