ArticleElectrophoresis2012
Comparing MALDI-MS, RP-LC-MALDI-MS and RP-LC-ESI-MS glycomic profiles of permethylated N-glycans derived from model glycoproteins and human blood serum.
Article in Electrophoresis, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- A mass spectrometry-based assay for mouse IgG N-glycan screening in biofluids.Analytical and bioanalytical chemistry · 2025Article
- Clinical glycoproteomics: methods and diseases.MedComm · 2024Review
- An Efficient and EconomicalMetabolites · 2022Article
- Exploring serum glycome patterns after moderate to severe traumatic brain injury: A prospective pilot study.EClinicalMedicine · 2022Article
- Glycome Profiling of Cancer Cell Lines Cultivated in Physiological and Commercial Media.Biomolecules · 2022Article
- Isomeric separation of permethylated glycans by extra-long reversed-phase liquid chromatography (RPLC)-MS/MS.The Analyst · 2022Article
- MS-based glycomics: An analytical tool to assess nervous system diseases.Frontiers in neuroscience · 2022Review
- Identification of Differential N-Glycan Compositions in the Serum and Tissue of Colon Cancer Patients by Mass Spectrometry.Biology · 2021Article
- Mass Spectrometry-Based Methods for Immunoglobulin G N-Glycosylation Analysis.Experientia supplementum (2012) · 2021Article
- 8-plex LC-MS/MS Analysis of PermethylatedAnalytical chemistry · 2019Article
- Bacteriophage T4 capsid as a nanocarrier for Peptide-N-Glycosidase F immobilization through self-assembly.Scientific reports · 2019Article
- LC-MS/MS glycomics of idiopathic rapid eye movement sleep behavior disorder.Electrophoresis · 2018Article
- Magnetic carbon nanocomposites as a MALDI co-matrix enhancing MS-based glycomics.Analytical and bioanalytical chemistry · 2018Article
- Application of network smoothing to glycan LC-MS profiling.Bioinformatics (Oxford, England) · 2018Article
- Carbon Nanoparticles and Graphene Nanosheets as MALDI Matrices in Glycomics: a New Approach to Improve Glycan Profiling in Biological Samples.Journal of the American Society for Mass Spectrometry · 2018Article
- Characterization of isomeric glycan structures by LC-MS/MS.Electrophoresis · 2017Review
- Quantitative Glycomics: A Combined Analytical and Bioinformatics Approach.Methods in enzymology · 2017Article
- Analysis of Permethylated Glycan by Liquid Chromatography (LC) and Mass Spectrometry (MS).Methods in molecular biology (Clifton, N.J.) · 2017Article
- Reversed-phase separation methods for glycan analysis.Analytical and bioanalytical chemistry · 2017Review
- Use of Mass Spectrometry to Screen Glycan Early Markers in Hepatocellular Carcinoma.Frontiers in oncology · 2017Review
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2 authors.
Funding
Abstract
The glycomic profiling of purified glycoproteins and biological specimen is routinely achieved through different analytical methods, but mainly through MS and LC-MS. The enhanced ionization efficiency and improved tandem MS interpretation of permethylated glycans have prompted the popularity of this approach. This study focuses on comparing the glycomic profiling of permethylated N-glycans derived from model glycoproteins and human blood serum using MALDI-MS as well as RP-LC-MALDI-MS and RP-LC-ESI-MS. In the case of model glycoproteins, the glycomic profiles acquired using the three methods were very comparable. However, this was not completely true in the case of glycans derived from blood serum. RP-LC-ESI-MS analysis of reduced and permethylated N-glycans derived from 250 nl of blood serum allowed the confident detection of 73 glycans (the structures of which were confirmed by mass accuracy and tandem MS), while 53 and 43 structures were identified in the case of RP-LC-MALDI-MS and MALDI-MS analyses of the same sample, respectively. RP-LC-ESI-MS analysis facilitates automated and sensitive tandem MS acquisitions. The glycan structures that were detected only in the RP-LC-ESI-MS analysis were glycans existing at low abundances. This is suggesting the higher detection sensitivity of RP-LC-ESI-MS analysis, originating from both reduced competitive ionization and saturation of detectors, facilitated by the chromatographic separation. The latter also permitted the separation of several structural isomers; however, isomeric separations pertaining to linkages were not detected.
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