ArticleThe Plant cell2012
Structure of soybean β-cyanoalanine synthase and the molecular basis for cyanide detoxification in plants.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 56 citations in OpenAlex.
- GhLYK5-mediated GhNIT4B activates asparagine and ROS accumulation enhancing cotton Verticillium wilt resistance.Journal of biological engineering · 2026Article
- Benzoic and salicylic acids inhibit β-substituted alanine synthase 4;1 in common bean.Plant physiology · 2025Article
- N-Terminal Metal-Binding Domain of Arabidopsis IBR5 Is Important for Its in Planta Functions.International journal of molecular sciences · 2025Article
- Horizontally acquired cysteine synthase genes undergo functional divergence in lepidopteran herbivores.Heredity · 2021Article
- Assimilation of exogenous cyanide cross talk in Oryza sativa L. to the key nodes in nitrogen metabolism.Ecotoxicology (London, England) · 2020Article
- Metabolism of the Cyanogenic Glucosides in Developing Flax: Metabolic Analysis, and Expression Pattern of Genes.Metabolites · 2020Article
- Ethylene Biosynthesis Inhibition Combined with Cyanide Degradation Confer Resistance to Quinclorac inInternational journal of molecular sciences · 2020Article
- Molecular cloning of putative chloroplastic cysteine synthase in Leucaena leucocephala.Journal of plant research · 2020Article
- Cyanide produced with ethylene by ACS and its incomplete detoxification by β-CAS in mango inflorescence leads to malformation.Scientific reports · 2019Article
- Comparative transcriptome analysis reveals significant differences in the regulation of gene expression between hydrogen cyanide- and ethylene-treated Arabidopsis thaliana.BMC plant biology · 2019Article
- Metabolomic analysis of date palm seedlings exposed to salinity and silicon treatments.Plant signaling & behavior · 2019Article
- β-N-Oxalyl-l-α,β-diaminopropionic Acid (β-ODAP) Content in Lathyrus sativus: The Integration of Nitrogen and Sulfur Metabolism through β-Cyanoalanine Synthase.International journal of molecular sciences · 2017Review
- A spinachBiochimie open · 2016Article
- Calcium Biofortification: Three Pronged Molecular Approaches for Dissecting Complex Trait of Calcium Nutrition in Finger Millet (Frontiers in plant science · 2016Review
- A gene horizontally transferred from bacteria protects arthropods from host plant cyanide poisoning.eLife · 2014Article
- Beyond toxicity: a regulatory role for mitochondrial cyanide.Plant signaling & behavior · 2014Article
- Structure of soybean serine acetyltransferase and formation of the cysteine regulatory complex as a molecular chaperone.The Journal of biological chemistry · 2013Article
- Transcriptome Profiling Identifies Candidate Genes Associated with the Accumulation of Distinct Sulfur γ-Glutamyl Dipeptides in Phaseolus vulgaris and Vigna mungo Seeds.Frontiers in plant science · 2013Article
- Collet Hairs Are Critical for Anchoring and Survival of Arabidopsis thaliana Seedlings During Early Development.Physiologia plantarumArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants produce cyanide (CN-) during ethylene biosynthesis in the mitochondria and require β-cyanoalanine synthase (CAS) for CN- detoxification. Recent studies show that CAS is a member of the β-substituted alanine synthase (BSAS) family, which also includes the Cys biosynthesis enzyme O-acetylserine sulfhydrylase (OASS), but how the BSAS evolved distinct metabolic functions is not understood. Here we show that soybean (Glycine max) CAS and OASS form α-aminoacrylate reaction intermediates from Cys and O-acetylserine, respectively. To understand the molecular evolution of CAS and OASS in the BSAS enzyme family, the crystal structures of Gm-CAS and the Gm-CAS K95A mutant with a linked pyridoxal phosphate (PLP)-Cys molecule in the active site were determined. These structures establish a common fold for the plant BSAS family and reveal a substrate-induced conformational change that encloses the active site for catalysis. Comparison of CAS and OASS identified residues that covary in the PLP binding site. The Gm-OASS T81M, S181M, and T185S mutants altered the ratio of OASS:CAS activity but did not convert substrate preference to that of a CAS. Generation of a triple mutant Gm-OASS successfully switched reaction chemistry to that of a CAS. This study provides new molecular insight into the evolution of diverse enzyme functions across the BSAS family in plants.
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