ArticleMedical science monitor : international medical journal of experimental and clinical research2012
Small interfering RNA-mediated downregulation of beta-catenin inhibits invasion and migration of colon cancer cells in vitro.
Article in Medical science monitor : international medical journal of experimental and clinical research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Importance of metastatic lymph node ratio in non-metastatic, lymph node-invaded colon cancer: a clinical trial.Medical science monitor : international medical journal of experimental and clinical research · 2014Trial
- Exosome-Mediated Paracrine Signaling Unveils miR-1246 as a Driver of Aggressiveness in Fusion-Negative Rhabdomyosarcoma.Cancers · 2024Article
- Preclinical and Clinical Research Models of Prostate Cancer: A Brief Overview.Life (Basel, Switzerland) · 2022Review
- Effects of differential distributed-JUP on the malignancy of gastric cancer.Journal of advanced research · 2021Article
- Combination of Inositol Hexaphosphate and Inositol Inhibits Liver Metastasis of Colorectal Cancer in Mice Through the Wnt/β-Catenin Pathway.OncoTargets and therapy · 2020Article
- Promotion of ovarian cancer cell invasion, migration and colony formation by the miR‑21/Wnt/CD44v6 pathway.Oncology reports · 2019Article
- Over-expression of SOX8 predicts poor prognosis in colorectal cancer: A retrospective study.Medicine · 2019Article
- Lichen-derived caperatic acid and physodic acid inhibit Wnt signaling in colorectal cancer cells.Molecular and cellular biochemistry · 2018Article
- 4,6,4'-trimethylangelicin shows high anti-proliferative activity on DU145 cells under both UVA and blue light.Cell proliferation · 2018Article
- Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes.International journal of oncology · 2018Article
- Knockdown of Tripartite Motif-Containing Protein 37 (TRIM37) Inhibits the Proliferation and Tumorigenesis in Colorectal Cancer Cells.Oncology research · 2017Article
- Pharmacologic Manipulation of Wnt Signaling and Cancer Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2017Review
- The role of ATP8A1 in non-small cell lung cancer.International journal of clinical and experimental pathology · 2017Article
- MicroRNA-200b Impacts Breast Cancer Cell Migration and Invasion by Regulating Ezrin-Radixin-Moesin.Medical science monitor : international medical journal of experimental and clinical research · 2016Article
- The Wnt signaling pathway in cancer.Critical reviews in oncology/hematology · 2016Review
- Molecular targets and pathways involved in liver metastasis of colorectal cancer.Clinical & experimental metastasis · 2015Review
- Tubeimoside-1 inhibits the growth and invasion of colorectal cancer cells through the Wnt/β-catenin signaling pathway.International journal of clinical and experimental pathology · 2015Article
- New type of BACE1 siRNA delivery to cells.Medical science monitor : international medical journal of experimental and clinical research · 2014Article
- Seasonality pattern of breast, colorectal, and prostate cancer is dependent on latitude.Medical science monitor : international medical journal of experimental and clinical research · 2014Article
- CD44 targets Wnt/β-catenin pathway to mediate the proliferation of K562 cells.Cancer cell international · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAbnormal regulation of Wnt/beta-catenin signaling and subsequently increased beta-catenin expression have been found to be involved in the proliferation and growth of colon cancer cells. Whether the down-regulation of beta-catenin in colon cancer may result in compromised invasion and migration in vitro still remains to be determined. MATERIAL/
methodsA human colon cancer cell line (LoVo cells) was transfected with small interfering RNA (siRNA) targeting beta-catenin. RT-PCR, Western blot assay, flow cytometry, cell adhesion assay, scratch wound assay, and matrigel invasion assay were performed, and the correlation between cell invasion and migration and beta-catenin expressions was analyzed.
resultssiRNA-mediated down-regulation of beta-catenin elevated the E-cadherin expression but reduced the MMP-7 and CD44v6 expressions, which increased the adhesion between LoVo cells but decreased the adhesion of LoVo cells to fibronectin. Significant inhibition of cell invasion and migration was also observed following RNA interference with beta-catenin siRNA.
conclusionssiRNA-mediated downregulation of beta-catenin could be valuable for defining gene expression and functional programs downstream of oncogenic beta-catenin signals, which, in turn, may be helpful to isolate novel diagnostic markers, and for designing tumor-specific intervention at downstream targets of oncogenic beta-catenin.
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