ArticleTissue engineering. Part A2012
Culture of primary bovine chondrocytes on a continuously expanding surface inhibits dedifferentiation.
Article in Tissue engineering. Part A, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Comparative assessment of human chondroprogenitor viability and molecular phenotype in various parenteral transport media.Histochemistry and cell biology · 2025Article
- Directed biomechanical compressive forces enhance fusion efficiency in model placental trophoblast cultures.Scientific reports · 2024Article
- Photo-Cross-Linkable, Injectable, and Highly Adhesive GelMA-Glycol Chitosan Hydrogels for Cartilage Repair.Advanced healthcare materials · 2023Article
- GelMA-glycol chitosan hydrogels for cartilage regeneration: The role of uniaxial mechanical stimulation in enhancing mechanical, adhesive, and biochemical properties.APL bioengineering · 2023Article
- Optimization of Protocol for Isolation of Chondrocytes from Human Articular Cartilage.Cartilage · 2021Article
- Feasibility of Human Platelet Lysate as an Alternative to Foetal Bovine Serum for In Vitro Expansion of Chondrocytes.International journal of molecular sciences · 2021Review
- Dedifferentiation: inspiration for devising engineering strategies for regenerative medicine.NPJ Regenerative medicine · 2020Review
- Articular Cartilage Aging-Potential Regenerative Capacities of Cell Manipulation and Stem Cell Therapy.International journal of molecular sciences · 2018Review
- Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Contribute to Chondrogenesis in Coculture with Chondrocytes.BioMed research international · 2016Article
- Past, present, and future of microcarrier-based tissue engineering.Journal of orthopaedic translation · 2015Review
- Enhancing chondrogenic phenotype for cartilage tissue engineering: monoculture and coculture of articular chondrocytes and mesenchymal stem cells.Tissue engineering. Part B, Reviews · 2014Review
- Prestress in the extracellular matrix sensitizes latent TGF-β1 for activation.The Journal of cell biology · 2014Article
- Effective cell collection method using collagenase and ultrasonic vibration.Biomicrofluidics · 2014Article
- Low-frequency high-magnitude mechanical strain of articular chondrocytes activates p38 MAPK and induces phenotypic changes associated with osteoarthritis and pain.International journal of molecular sciences · 2014Article
- A novel stretching platform for applications in cell and tissue mechanobiology.Journal of visualized experiments : JoVE · 2014Article
- Articular chondrocyte redifferentiation in 3D co-cultures with mesenchymal stem cells.Tissue engineering. Part C, Methods · 2014Article
- High mechanical strain of primary intervertebral disc cells promotes secretion of inflammatory factors associated with disc degeneration and pain.Arthritis research & therapy · 2014Article
- A copper sulfate and hydroxylysine treatment regimen for enhancing collagen cross-linking and biomechanical properties in engineered neocartilage.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- P38 mitogen-activated protein kinase promotes dedifferentiation of primary articular chondrocytes in monolayer culture.Journal of cellular and molecular medicine · 2013Article
- Maintenance of chondrocyte phenotype during expansion on PLLA microtopographies.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Expansion of autologous chondrocytes in vitro is used to generate adequate populations for cell-based therapies. However, standard (SD) culture methods cause loss of chondrocyte phenotype and dedifferentiation to fibroblast-like cells. Here, we use a novel surface expansion culture system in an effort to inhibit chondrocyte dedifferentiation. A highly elastic silicone rubber culture surface was continuously stretched over a 13-day period to 600% of its initial surface area. This maintained cells at a high density while limiting contact inhibition and reducing the need for passaging. Gene expression analysis, biochemical assays, and immunofluorescence microscopy of follow-on pellet cultures were used to characterize the results of continuous expansion (CE) culture versus SD cultures on rigid polystyrene. CE culture yielded cells with a more chondrocyte-like morphology and higher RNA-level expression of the chondrogenic markers collagen type II, aggrecan, and cartilage oligomeric matrix protein. Furthermore, the expression of collagen type I RNA and α-smooth muscle actin protein were significantly reduced, indicating suppression of fibroblastic features. Pellet cultures from CE chondrocytes contained more sulphated glycosaminoglycan and collagen type II than pellets from SD culture. Additional control cultures on static (unexpanded) silicone (SS culture) indicated that benefits of CE culture were partially due to features of the culture surface itself and partially due to the reduced passaging which that surface enabled through CE. Chondrocytes grown in CE culture may, therefore, be a superior source for cell-based therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.