ArticleIranian Red Crescent medical journal2011
Detection of drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of iran.
Article in Iranian Red Crescent medical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.
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Who cites it
8 citing papers in PubMed, 1 synthesis or guideline pooled it, 16 citations in OpenAlex.
- Leishmania spp. genetic factors associated with cutaneous leishmaniasis antimony pentavalent drug resistance: a systematic review.Memorias do Instituto Oswaldo Cruz · 2024Pooled it
- Investigating the Effect of Satureja khuzestanica Essential oil on MDR1 Gene Expression in Leishmania major.Acta parasitologica · 2024Article
- High resolution melting analysis and detection of Leishmania resistance: the role of multi drug resistance 1 gene.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021Article
- Prediction ofGalen medical journal · 2018Article
- Treatment Outcome of the Drug-resistant Zoonotic Cutaneous Leishmaniasis by Glucantime.Advanced biomedical research · 2017Article
- Attenuation and Production of the Amphotericin B-Resistant Leishmania tropica Strain.Jundishapur journal of microbiology · 2016Article
- A New Antileishmanial Preparation of Combined Solamargine and Solasonine Heals Cutaneous Leishmaniasis through Different Immunochemical Pathways.Antimicrobial agents and chemotherapy · 2016Article
- P-glycoprotein A Gene Expression in Glucantime-Resistant and Sensitive Leishmania major (MRHO/IR/75/ER).Iranian journal of parasitology · 2014Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCutaneous leishmaniasis is still a health problem in many rural and urban regions of Iran and drug resistance has emerged as a major impediment in the treatment of leishmaniasis. This study aims to determine the drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran.
methodsNinety seven samples were collected from ulcers of leishmaniasis patients from some endemic areas of Iran. The Giemsa stained samples were examined microscopically and cultured in NNN and RPMI 1640 mediums for parasite detection. After DNA extraction, PCR was done by a pair of specific primers. For detection of mutation in DNA, first PCR products were electrophoresed on CSGE gel. The suspected samples were compared by sequencing and RFLP results were demonstrated. Comparison of DNA derived from a wild type cell and mutant cell was undertaken by CSGE and sequencing methods.
resultsAmong 90 isolates (92.8%) examined for detection of mutation in gene with CSGE and RFLP, 10 (11.1%) revealed a disorder in sequencing selection for unresponsive to drug.
conclusionDrug resistance in cutaneous leishmaniasis to sodium stiboglocanat is probably due to a mutation in a genome. A field study is needed to determine the distribution of drug resistance and other gene mutations involved in unresponsiveness to drugs in leishmaniasis endemic areas of Iran.
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Identifiers
22737430PMC3371903W2119417386What OpenQuestion holds
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