ArticlePloS one2012
Actin polymerization controls the organization of WASH domains at the surface of endosomes.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 71 citations in OpenAlex.
- Hybrid endosomal coats contain different classes of sorting nexins.The EMBO journal · 2026Article
- Endosomal actin attenuation and fission are regulated by MICAL2.Journal of cell science · 2026Article
- MFF budding from mitochondria regulates melanosome size and maturation.Nature communications · 2026Article
- Identification of a RAB32-LRMDA-Commander membrane trafficking complex reveals the molecular mechanism of human oculocutaneous albinism type 7.Nature communications · 2025Article
- CCDC22 mutations that impair COMMD binding cause attenuated 3C/Ritscher-Schinzel syndrome.BMC medical genomics · 2025Article
- SNX5 promotes antigen presentation in B cells by dual regulation of actin and lysosomal dynamics.Life science alliance · 2025Article
- Endosomal actin branching, fission, and receptor recycling require FCHSD2 recruitment by MICAL-L1.Molecular biology of the cell · 2024Article
- Assembly and fission of tubular carriers mediating protein sorting in endosomes.Nature reviews. Molecular cell biology · 2024Review
- Mechanism and regulation of cargo entry into the Commander endosomal recycling pathway.Nature communications · 2024Article
- IST1 regulates select recycling pathways.Traffic (Copenhagen, Denmark) · 2024Article
- Codependencies of mTORC1 signaling and endolysosomal actin structures.Science advances · 2023Article
- Receptor Recycling by Retromer.Molecular and cellular biology · 2023Review
- Coronin2A links actin-based endosomal processes to the EHD1 fission machinery.Molecular biology of the cell · 2022Article
- Opposing functions for retromer and Rab11 in extracellular vesicle traffic at presynaptic terminals.The Journal of cell biology · 2021Article
- The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex.Science advances · 2021Article
- Assembly and Activity of the WASH Molecular Machine: Distinctive Features at the Crossroads of the Actin and Microtubule Cytoskeletons.Frontiers in cell and developmental biology · 2021Review
- Review
- Ebola Virus Nucleocapsid-Like Structures Utilize Arp2/3 Signaling for Intracellular Long-Distance Transport.Cells · 2020Article
- To degrade or not to degrade: mechanisms and significance of endocytic recycling.Nature reviews. Molecular cell biology · 2018Review
- Expression of N471D strumpellin leads to defects in the endolysosomal system.Disease models & mechanisms · 2018Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sorting of cargoes in endosomes occurs through their selective enrichment into sorting platforms, where transport intermediates are generated. The WASH complex, which directly binds to lipids, activates the Arp2/3 complex and hence actin polymerization onto such sorting platforms. Here, we analyzed the role of actin polymerization in the physiology of endosomal domains containing WASH using quantitative image analysis. Actin depolymerization is known to enlarge endosomes. Using a novel colocalization method that is insensitive to the heterogeneity of size and shape of endosomes, we further show that preventing the generation of branched actin networks induces endosomal accumulation of the WASH complex. Moreover, we found that actin depolymerization induces a dramatic decrease in the recovery of endosomal WASH after photobleaching. This result suggests a built-in turnover, where the actin network, i.e. the product of the WASH complex, contributes to the dynamic exchange of the WASH complex by promoting its detachment from endosomes. Our experiments also provide evidence for a role of actin polymerization in the lateral compartmentalization of endosomes: several WASH domains exist at the surface of enlarged endosomes, however, the WASH domains coalesce upon actin depolymerization or Arp2/3 depletion. Branched actin networks are thus involved in the regulation of the size of WASH domains. The potential role of this regulation in membrane scission are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.