ArticleThe Journal of biological chemistry2012
A mechanism of intracellular P2X receptor activation.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- VPS8D, a CORVET subunit, is required to maintain the contractile vacuole complex inbioRxiv : the preprint server for biology · 2023Article
- The old and the new about the contractile vacuole of Trypanosoma cruzi.The Journal of eukaryotic microbiology · 2022Review
- Matrix metalloproteinases, purinergic signaling, and epigenetics: hubs in the spinal neuroglial network following peripheral nerve injury.Histochemistry and cell biology · 2022Article
- A polycystin-type transient receptor potential (Trp) channel that is activated by ATP.Biology open · 2017Article
- Comparative study of the P2X gene family in animals and plants.Purinergic signalling · 2016Article
- Evidence for Extracellular ATP as a Stress Signal in a Single-Celled Organism.Eukaryotic cell · 2015Article
- P2X4 forms functional ATP-activated cation channels on lysosomal membranes regulated by luminal pH.The Journal of biological chemistry · 2014Article
- Rab11 regulates trafficking of trans-sialidase to the plasma membrane through the contractile vacuole complex of Trypanosoma cruzi.PLoS pathogens · 2014Article
- Calcium-dependent regulation of Rab activation and vesicle fusion by an intracellular P2X ion channel.Nature cell biology · 2014Article
- Primitive ATP-activated P2X receptors: discovery, function and pharmacology.Frontiers in cellular neuroscience · 2013Review
- ATP-Binding Cassette Transporter B4 Anchors the Cell Adhesion Molecule DdCAD-1 to Cell Membrane in Dictyostelium discoideum.Indian journal of microbiology · 2013Article
- The trafficking and targeting of P2X receptors.Frontiers in cellular neuroscience · 2013Review
- Functional properties of five Dictyostelium discoideum P2X receptors.The Journal of biological chemistry · 2013Article
- Effect of P2X4 and P2X7 receptor antagonism on the pressure diuresis relationship in rats.Frontiers in physiology · 2013Article
- New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists.International review of cell and molecular biology · 2013Review
- Intracellular P2X receptors as novel calcium release channels and modulators of osmoregulation in Dictyostelium: a comparison of two common laboratory strains.Channels (Austin, Tex.) · 2013Article
- Cloning and characterization of a P2X receptor expressed in the central nervous system of Lymnaea stagnalis.PloS one · 2012Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
P2X receptors (P2XRs) are ATP-activated calcium-permeable ligand-gated ion channels traditionally viewed as sensors of extracellular ATP during diverse physiological processes including pain, inflammation, and taste. However, in addition to a cell surface residency P2XRs also populate the membranes of intracellular compartments, including mammalian lysosomes, phagosomes, and the contractile vacuole (CV) of the amoeba Dictyostelium. The function of intracellular P2XRs is unclear and represents a major gap in our understanding of ATP signaling. Here, we exploit the genetic versatility of Dictyostelium to investigate the effects of physiological concentrations of ATP on calcium signaling in isolated CVs. Within the CV, an acidic calcium store, P2XRs are orientated to sense luminal ATP. Application of ATP to isolated vacuoles leads to luminal translocation of ATP and release of calcium. Mechanisms of luminal ATP translocation and ATP-evoked calcium release share common pharmacology, suggesting that they are linked processes. The ability of ATP to mobilize stored calcium is reduced in vacuoles isolated from P2X(A)R knock-out amoeba and ablated in cells devoid of P2XRs. Pharmacological inhibition of luminal ATP translocation or depletion of CV calcium attenuates CV function in vivo, manifesting as a loss of regulatory cell volume decrease following osmotic swelling. We propose that intracellular P2XRs regulate vacuole activity by acting as calcium release channels, activated by translocation of ATP into the vacuole lumen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.