ArticleEuropean journal of immunology2012
Cytotoxic T lymphocyte effector function is independent of nucleus-centrosome dissociation.
Article in European journal of immunology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- The role of protease-activated receptor 1 signaling in CD8 T cell effector functions.iScience · 2021Article
- The Bardet-Biedl syndrome complex component BBS1 controls T cell polarity during immune synapse assembly.Journal of cell science · 2021Article
- Search and Capture Efficiency of Dynamic Microtubules for Centrosome Relocation during IS Formation.Biophysical journal · 2019Article
- Nuclear morphologies: their diversity and functional relevance.Chromosoma · 2017Review
- Centrosome positioning in non-dividing cells.Protoplasma · 2016Review
- Article
- The role of the cytoskeleton at the immunological synapse.Immunological reviews · 2013Review
- Centrosome repositioning in T cells is biphasic and driven by microtubule end-on capture-shrinkage.The Journal of cell biology · 2013Article
- Cell polarisation and the immunological synapse.Current opinion in cell biology · 2013Review
- Nuclear envelope lamin-A as a coordinator of T cell activation.Nucleus (Austin, Tex.)Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Cytotoxic T lymphocytes (CTLs) kill tumorigenic and virally infected cells by targeted secretion of lytic granule contents. The precise point at which secretion occurs is directed by the centrosome docking at the immunological synapse (IS). The centrosome is highly dynamic in CTLs, lagging behind the nucleus in the uropod of migrating CTLs, but translocating across the entire length of the cell to dock at the IS when a target cell is recognized. While in most cell types, the centrosome is always closely associated with the nuclear membrane, in CTLs, it often appears to be dissociated from the nucleus, both in migrating cells and when forming an IS. We asked whether this dissociation is required for CTL killing, by expressing GFP-BICD2-NT-nesprin-3, which tethers the centrosome to the nucleus irreversibly. Immunofluorescence microscopy revealed that the centrosome polarized successfully to the central supramolecular activation complex (cSMAC) of the synapse in GFP-BICD2-NT-nesprin-3-expressing CTLs, with the centrosome and nucleus migrating together to the IS. CTLs in which the centrosome was "glued" to the nucleus were able to dock and release granules at the IS as effectively as mock-treated cells. These data demonstrate that CTL cytotoxicity is independent of centrosomal dissociation from the nuclear envelope.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.