Evidence map›Paper›PMID 22736111›Full record

ArticleMolecular biology reports2012

The effects of PDE5 inhibitory drugs on renal ischemia/reperfusion injury in rats.

A Küçük, M Yucel, N Erkasap, M Tosun, T Koken, M Ozkurt, S Erkasap

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 51 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Reno-protective effects of Phosphodiesterase 5 inhibitors.Clinical and experimental nephrology · 2021
    Review
  5. Review
  6. Tadalafil attenuates ischemic damage as well as reperfusion injury in the rat ovaryJournal of the Turkish German Gynecological Association · 2020
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Sildenafil Prevents Podocyte InjuryJournal of the American Society of Nephrology : JASN · 2017
    Article
  12. Phosphodiesterase type 5 inhibitors and kidney disease.International urology and nephrology · 2015
    Review
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

A KüçükDepartment of Physiology, Medical Faculty, Dumlupinar University, Central Campus, Tavsanlı Road 10.km, 43270 Kutahya, Turkey. kucukaysegul@hotmail.com
M Yucel
N Erkasap
M Tosun
T Koken
M Ozkurt
S Erkasap
Eskişehir Osmangazi University · TRAfyon Kocatepe University · TRDumlupinar University · TR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of the present study was to evaluate the effects of phosphodiesterase type 5 (PDE5) inhibitory drugs, Tadalafil and Sildenafil, on inducible NOS (iNOS), endothelial NOS (eNOS) and p53 genes expressions and apoptosis in ischemia/reperfusion (I/R) induced oxidative injury in rat renal tissue. Eighty Sprague-Dawley rats (300-350 g) were divided into four groups. In ischemia/reperfusion group, rats were subjected to renal ischemia by clamping the left pedicle for 60 min, and then reperfused for 90 min. On the other hand, in other two groups the rats were individually pretreated with Tadalafil and Sildenafil 1 h before the induction of ischemia. Malondialdehyde (MDA) is determined in renal tissue homogenates by high-performance liquid chromatography, the number of apoptotic cell were calculated by TUNEL method and p53 and eNOS expression were detected with immunohistochemistry. On the other hand, myeloperoxidase (MPO) levels were measured by spectrophotometric method and the mRNA level of iNOS in renal tissue was determined by Real-time PCR (RT-PCR). Our results indicate that MDA and MPO levels were increased in the I/R group than those in the control group. Both Tadalafil and Sildenafil treatment decreased the MDA levels in ischemia/reperfusion group, whereas this effect was more potent with Sildenafil. RT-PCR results showed that, iNOS gen expression increased in the I/R group, but decreased in the PDE5 inhibitory drugs treated group. Apoptotic cells, eNOS levels and p53 positive cells were also decreased in PDE5 inhibitory drugs treated group. We suggest that Tadalafil and Sildenafil have beneficial effects against I/R related renal tissue injury and this protective effect is clearer for Sildenafil than Tadalafil.

Indexed as

AnimalsApoptosisCarbolinesGene ExpressionIschemiaKidneyMaleMalondialdehydeNitric Oxide Synthase Type IINitric Oxide Synthase Type IIIOxidative StressPeroxidasePhosphodiesterase 5 InhibitorsPiperazinesPurinesRatsCarbolinesMalondialdehydeNitric Oxide Synthase Type IINitric Oxide Synthase Type IIINos2 protein, ratNos3 protein, ratPeroxidasePhosphodiesterase 5 InhibitorsPiperazinesPurinesSildenafil CitrateSulfonesTadalafilTumor Suppressor Protein p53

Identifiers

PMID22736111
OpenAlexW2023965118

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.