ArticleMethods in molecular biology (Clifton, N.J.)2012
Ribosome purification approaches for studying interactions of regulatory proteins and RNAs with the ribosome.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Article
- Ribosome hibernation in zinc-starvedMicrobiology spectrum · 2026Article
- Ribosome RNA Extraction and RNase R Treatment.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation.Molecular cell · 2025Article
- A novelProceedings of the National Academy of Sciences of the United States of America · 2024Article
- RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages.Molecular cell · 2024Article
- RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages.bioRxiv : the preprint server for biology · 2023Article
- Lamotrigine compromises the fidelity of initiator tRNA recruitment to the ribosomal P-site by IF2 and the RbfA release from 30S ribosomes inRNA biology · 2023Article
- Structural insights into the human PA28-20S proteasome enabled by efficient tagging and purification of endogenous proteins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The Ribosome Is the Ultimate Receptor for Trypsin Modulating Oostatic Factor (TMOF).Biomolecules · 2022Article
- Effect of Amino Acid Substitutions on 70S Ribosomal Binding, Cellular Uptake, and Antimicrobial Activity of Oncocin Onc112.Chembiochem : a European journal of chemical biology · 2022Article
- Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance.eLife · 2022Article
- Recombinant Expression and Purification of Large Bacterial Multienzyme Assemblies for Biosynthetic Processes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Toeprint Assays for Detecting RNA Structure and Protein-RNA Interactions.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitro.RNA (New York, N.Y.) · 2021Article
- miR-16-5p Promotes Erythroid Maturation of Erythroleukemia Cells by Regulating Ribosome Biogenesis.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Oxidative Damage to RNA is Altered by the Presence of Interacting Proteins or Modified Nucleosides.Frontiers in molecular biosciences · 2021Article
- Role of Hibernation Promoting Factor in Ribosomal Protein Stability duringInternational journal of molecular sciences · 2020Article
- Grad-seq shines light on unrecognized RNA and protein complexes in the model bacterium Escherichia coli.Nucleic acids research · 2020Article
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Authors and funding
4 authors.
Funding
Abstract
Ribosomes are large complexes of RNA and protein that perform the essential task of protein synthesis in the cell. Ribosomes also serve as the initiation point for several translation-associated functions. To perform these tasks efficiently, ribosomes interact with a myriad of nonribosomal proteins and RNAs. Given that most of these interactions are transient, purification of the interacting factors in complex with the ribosome can be a challenging undertaking. Here, we review methods commonly used to isolate ribosomes and study ribosome-associated factors. We also discuss crucial parameters for designing and executing ribosome association studies. Finally, we present a detailed protocol for reporter based enrichment assays that are employed to selectively isolate ribosomes translating a particular message of interest. These protocols can be used to study a wide range of ribosome-associated functions.
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