ArticleBiophysical journal2012
STED microscopy with optimized labeling density reveals 9-fold arrangement of a centriole protein.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
- Critical constituents and assembly principles of centriole biogenesis in human cells.Nature reviews. Molecular cell biology · 2026Review
- Insights into the zebrafish left-right organizer's centrosomes and cilia via volume electron microscopy.Biology open · 2026Article
- Centriole Duplication, Maturation, and Transformation During Mammalian Spermatogenesis.Advances in experimental medicine and biology · 2026Review
- Advances in high-resolution imaging of centrioles and cilia.Journal of cell science · 2025Review
- Review
- Analysis of immune synapses by τau-STED imaging and 3D-quantitative colocalization of lytic granule markers.Methods in cell biology · 2025Article
- Iterative Immunostaining and NEDD Denoising for Improved Signal-To-Noise Ratio in ExM-LSCM.Bio-protocol · 2024Article
- Article
- Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding.Science translational medicine · 2024Article
- Review
- Iterative immunostaining combined with expansion microscopy and image processing reveals nanoscopic network organization of nuclear lamina.Molecular biology of the cell · 2023Article
- Label-free nanoscale mapping of intracellular organelle chemistry.Communications biology · 2023Article
- Single-molecule localization microscopy reveals the ultrastructural constitution of distal appendages in expanded mammalian centrioles.Nature communications · 2023Article
- Single-molecule imaging in the primary cilium.Methods in cell biology · 2023Article
- Super-Resolution Imaging of Peroxisomal Proteins Using STED Nanoscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Arf-like Protein 2 (ARL2) Controls Microtubule Neogenesis during Early Postnatal Photoreceptor Development.Cells · 2022Article
- Postmitotic centriole disengagement and maturation leads to centrosome amplification in polyploid trophoblast giant cells.Molecular biology of the cell · 2022Article
- Deletion of CEP164 in mouse photoreceptors post-ciliogenesis interrupts ciliary intraflagellar transport (IFT).PLoS genetics · 2022Article
- 3D-Structured Illumination Microscopy of Centrosomes in Human Cell Lines.Bio-protocol · 2022Article
- Super-resolution microscopy reveals photoreceptor-specific subciliary location and function of ciliopathy-associated protein CEP290.JCI insight · 2021Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
Super-resolution fluorescence microscopy can achieve resolution beyond the optical diffraction limit, partially closing the gap between conventional optical imaging and electron microscopy for elucidation of subcellular architecture. The centriole, a key component of the cellular control and division machinery, is 250 nm in diameter, a spatial scale where super-resolution methods such as stimulated emission depletion (STED) microscopy can provide previously unobtainable detail. We use STED with a resolution of 60 nm to demonstrate that the centriole distal appendage protein Cep164 localizes in nine clusters spaced around a ring of ∼300 nm in diameter, and quantify the influence of the labeling density in STED immunofluorescence microscopy. We find that the labeling density dramatically influences the observed number, size, and brightness of labeled Cep164 clusters, and estimate the average number of secondary antibody labels per cluster. The arrangements are morphologically similar in centrioles of both proliferating cells and differentiated multiciliated cells, suggesting a relationship of this structure to function. Our STED measurements in single centrioles are consistent with results obtained by electron microscopy, which involve ensemble averaging or very different sample preparation conditions, suggesting that we have arrived at a direct measurement of a centriole protein by careful optimization of the labeling density.
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