ArticleBiophysical journal2012
Activation thermodynamics of poly(ethylene glycol)-mediated model membrane fusion support mechanistic models of stalk and pore formation.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
- The Conserved Fusion Peptide Sequence of SARS-CoVs is Insensitive to CaThe Journal of membrane biology · 2026Article
- The Interplay of Lipids and Peptides in Membrane Merger.Advances in experimental medicine and biology · 2026Review
- Dengue Virus Fusion Peptide Promotes Hemifusion Formation by Disordering the Interfacial Region of the Membrane.The Journal of membrane biology · 2025Article
- Mechanisms of SNARE proteins in membrane fusion.Nature reviews. Molecular cell biology · 2024Review
- Cr(vi) permanently binds to the lipid bilayer in an inverted hexagonal phase throughout the reduction process.RSC advances · 2023Article
- Two modes of fusogenic action for influenza virus fusion peptide.PLoS computational biology · 2023Article
- Lipid and Lipidation in Membrane Fusion.The Journal of membrane biology · 2022Review
- Mechanism of Membrane Fusion: Interplay of Lipid and Peptide.The Journal of membrane biology · 2022Review
- Calcium-triggered fusion of lipid membranes is enabled by amphiphilic nanoparticles.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Membrane Composition Modulates Fusion by Altering Membrane Properties and Fusion Peptide Structure.The Journal of membrane biology · 2019Review
- Fluorescence-based ion sensing in lipid membranes: a simple method of sensing in aqueous medium with enhanced efficiency.RSC advances · 2019Article
- Exocytotic fusion pores are composed of both lipids and proteins.Nature structural & molecular biology · 2016Article
- Phosphatidylserine-Dependent Catalysis of Stalk and Pore Formation by Synaptobrevin JMR-TMD Peptide.Biophysical journal · 2015Article
- Coupled diffusion in lipid bilayers upon close approach.Journal of the American Chemical Society · 2015Article
- pH Alters PEG-mediated fusion of phosphatidylethanolamine-containing vesicles.Biophysical journal · 2014Article
- The transmembrane domain peptide of vesicular stomatitis virus promotes both intermediate and pore formation during PEG-mediated vesicle fusion.Biophysical journal · 2014Article
- Wild-type and mutant hemagglutinin fusion peptides alter bilayer structure as well as kinetics and activation thermodynamics of stalk and pore formation differently: mechanistic implications.Biophysical journal · 2013Article
- A novel assay for detecting fusion pore formation: implications for the fusion mechanism.Biochemistry · 2013Article
- Importance of the hexagonal lipid phase in biological membrane organization.Frontiers in plant science · 2013Article
- Phosphatidylserine inhibits and calcium promotes model membrane fusion.Biophysical journal · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Membrane fusion, essential to eukaryotic life, is broadly envisioned as a three-step process proceeding from contacting bilayers through two semistable, nonlamellar lipidic intermediate states to a fusion pore. Here, we introduced a new, to our knowledge, experimental approach to gain insight into the nature of the transition states between initial, intermediate, and final states. Recorded time courses of lipid-mixing, content-mixing, and content-leakage associated with fusion of 23 nm vesicles in the presence of poly(ethylene glycol) at multiple temperatures were fitted globally to a three-step sequential model to yield rate constants and thereby activation thermodynamics for each step of the process, as well as probabilities of occurrence of lipid-mixing, content-mixing, or content-leakage in each state. Experiments with membranes containing hexadecane, known to reduce interstice energy in nonlamellar structures, provided additional insight into the nature of fusion intermediates and transition states. The results support a hypothesis for the mechanism of stalk formation (step-1) that involves acyl chain protrusions into the interbilayer contact region, a hypothesis for a step-2 mechanism involving continuous interconversion of semistable nonlamellar intermediates, and a hypothesis for step-3 (pore formation) mechanism involving correlated movement of whole lipid molecules into hydrophobic spaces created by geometry mismatch between intermediate structures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.