ArticleChronobiology international2012
Fibroblast circadian rhythms of PER2 expression depend on membrane potential and intracellular calcium.
Article in Chronobiology international, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Circadian regulation of extracellular vesicle biogenesis, composition, and release.Npj biological timing and sleep · 2025Review
- Liver as a nexus of daily metabolic cross talk.International review of cell and molecular biology · 2025Review
- mTORC1 induces plasma membrane depolarization and promotes preosteoblast senescence by regulating the sodium channel Scn1a.Bone research · 2022Article
- Sleep Problems in Chronic Inflammatory Diseases: Prevalence, Treatment, and New Perspectives: A Narrative Review.Journal of clinical medicine · 2021Review
- Circadian rhythms in bipolar disorder patient-derived neurons predict lithium response: preliminary studies.Molecular psychiatry · 2021Article
- Communicating clocks shape circadian homeostasis.Science (New York, N.Y.) · 2021Review
- Contraction influences Per2 gene expression in skeletal muscle through a calcium-dependent pathway.The Journal of physiology · 2020Article
- Eukaryotic cell biology is temporally coordinated to support the energetic demands of protein homeostasis.Nature communications · 2020Article
- Article
- Calcium Sets the Clock in Ameloblasts.Frontiers in physiology · 2020Article
- A Method for Culturing Mouse Whisker Follicles to Study Circadian RhythmsBio-protocol · 2019Article
- Maternal eating behavior is a major synchronizer of fetal and postnatal peripheral clocks in mice.Cellular and molecular life sciences : CMLS · 2018Article
- Emerging Roles of the Membrane Potential: Action Beyond the Action Potential.Frontiers in physiology · 2018Review
- A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans.Scientific reports · 2017Article
- Lithium effects on circadian rhythms in fibroblasts and suprachiasmatic nucleus slices from Cry knockout mice.Neuroscience letters · 2016Article
- Calcium channel genes associated with bipolar disorder modulate lithium's amplification of circadian rhythms.Neuropharmacology · 2016Article
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- Article
- Fibroblast PER2 circadian rhythmicity depends on cell density.Journal of biological rhythms · 2013Article
- Mis-expression of the BK K(+) channel disrupts suprachiasmatic nucleus circuit rhythmicity and alters clock-controlled behavior.American journal of physiology. Cell physiology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The suprachiasmatic nucleus (SCN) of the hypothalamus synchronizes circadian rhythms of cells and tissues throughout the body. In SCN neurons, rhythms of clock gene expression are suppressed by manipulations that hyperpolarize the plasma membrane or lower intracellular Ca(2+). However, whether clocks in other cells also depend on membrane potential and calcium is unknown. In this study, the authors investigate the effects of membrane potential and intracellular calcium on circadian rhythms in mouse primary fibroblasts. Rhythms of clock gene expression were monitored using a PER2::LUC knockin reporter. Rhythms were lost or delayed at lower (hyperpolarizing) K(+) concentrations. Bioluminescence imaging revealed that this loss of rhythmicity in cultures was due to loss of rhythmicity of single cells rather than loss of synchrony among cells. In lower Ca(2+) concentrations, rhythms were advanced or had shorter periods. Buffering intracellular Ca(2+) by the calcium chelator 1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis acetoxymethyl ester (BAPTA-AM) or manipulation of inositol triphosphate (IP(3))-sensitive intracellular calcium stores by thapsigargin delayed rhythms. These results suggest that the circadian clock in fibroblasts, as in SCN neurons, is regulated by membrane potential and Ca(2+). Changes in intracellular Ca(2+) may mediate the effects of membrane potential observed in this study.
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Registered trials
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