ArticleMolecular and cellular biology2012
A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling.
Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Caspase-4 promotes metastasis and interferon-γ-induced pyroptosis in lung adenocarcinoma.Communications biology · 2024Article
- Allele-specific endogenous tagging and quantitative analysis of β-catenin in colorectal cancer cells.eLife · 2022Article
- Ykt6-dependent endosomal recycling is required for Wnt secretion in theDevelopment (Cambridge, England) · 2020Article
- DHEA Inhibits Leukocyte Recruitment through Regulation of the Integrin Antagonist DEL-1.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- CASP4 gene silencing in epithelial cancer cells leads to impairment of cell migration, cell-matrix adhesion and tissue invasion.Scientific reports · 2018Article
- Toward an integrated map of genetic interactions in cancer cells.Molecular systems biology · 2018Article
- ERAD-dependent control of the Wnt secretory factor Evi.The EMBO journal · 2018Article
- RNAi-Based Identification of Gene-Specific Nuclear Cofactor Networks Regulating Interleukin-1 Target Genes.Frontiers in immunology · 2018Article
- ENCoRE: an efficient software for CRISPR screens identifies new players in extrinsic apoptosis.BMC genomics · 2017Article
- Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Comprehensive analysis of high-throughput screens with HiTSeekR.Nucleic acids research · 2016Article
- Transcriptome Profiling Reveals the Antitumor Mechanism of Polysaccharide from Marine Algae Gracilariopsis lemaneiformis.PloS one · 2016Article
- The Power Decoder Simulator for the Evaluation of Pooled shRNA Screen Performance.Journal of biomolecular screening · 2015Article
- Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening.Genome medicine · 2015Article
- The role of death-associated protein 3 in apoptosis, anoikis and human cancer.Cancer cell international · 2015Article
- NF-κB regulates caspase-4 expression and sensitizes neuroblastoma cells to Fas-induced apoptosis.PloS one · 2015Article
- A critical role for human caspase-4 in endotoxin sensitivity.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Characterization of a novel necrotic granuloma model of latent tuberculosis infection and reactivation in mice.The American journal of pathology · 2014Article
- NF-κB-modulated miR-130a targets TNF-α in cervical cancer cells.Journal of translational medicine · 2014Article
- Small interfering RNA-based molecular therapy of cancers.Chinese journal of cancer · 2013Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tumor necrosis factor alpha (TNF-α) is a potent inflammatory cytokine secreted upon cellular stress as well as immunological stimuli and is implicated in the pathology of inflammatory diseases and cancer. The therapeutic potential of modifying TNF-α pathway activity has been realized in several diseases, and antagonists of TNF-α have reached clinical applications. While much progress in the understanding of signaling downstream of the TNF-α receptor complex has been made, the compendium of factors required for signal transduction is still not complete. In order to find novel regulators of proinflammatory signaling induced by TNF-α, we conducted a genome-wide small interfering RNA screen in human cells. We identified several new candidate modulators of TNF-α signaling, which were confirmed in independent experiments. Specifically, we show that caspase 4 is required for the induction of NF-κB activity, while it appears to be dispensable for the activation of the Jun N-terminal protein kinase signaling branch. Taken together, our experiments identify caspase 4 as a novel regulator of TNF-α-induced NF-κB signaling that is required for the activation of IκB kinase. We further provide the genome-wide RNA interference data set as a compendium in a format compliant with minimum information about an interfering RNA experiment (MAIRE).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.