ArticleMolecular and cellular biology2012
Alternative splicing regulates Prdm1/Blimp-1 DNA binding activities and corepressor interactions.
Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- PRDM1 restricts bladder cancer progression and enhances chemosensitivity by suppressing OTUD6A-mediated deubiquitination of CDC6.Cell death & disease · 2026Article
- Exploring the Use of Alternative Promoters for Enhanced Transgene and sgRNA Expression in Atlantic Salmon Cells.Marine biotechnology (New York, N.Y.) · 2024Article
- Massively parallel knock-in engineering of human T cells.Nature biotechnology · 2023Article
- A System for In Vitro Generation of Mature Murine Plasma Cells Uncovers DifferentialJournal of immunology (Baltimore, Md. : 1950) · 2022Article
- PD-1 Expression during Acute Infection Is Repressed through an LSD1-Blimp-1 Axis.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- Requirements for Neurogenin2 during mouse postnatal retinal neurogenesis.Developmental biology · 2018Article
- Blimp-1/PRDM1 is a critical regulator of Type III Interferon responses in mammary epithelial cells.Scientific reports · 2018Article
- Prdm1 Regulates Thymic Epithelial Function To Prevent Autoimmunity.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Blimp1/Prdm1 Functions in Opposition to Irf1 to Maintain Neonatal Tolerance during Postnatal Intestinal Maturation.PLoS genetics · 2015Article
- Transcriptional and epigenetic networks of helper T and innate lymphoid cells.Immunological reviews · 2014Review
- The PR/SET domain zinc finger protein Prdm4 regulates gene expression in embryonic stem cells but plays a nonessential role in the developing mouse embryo.Molecular and cellular biology · 2013Article
- PRDM Proteins: Molecular Mechanisms in Signal Transduction and Transcriptional Regulation.Biology · 2013Article
- IFN-α regulates Blimp-1 expression via miR-23a and miR-125b in both monocytes-derived DC and pDC.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Prdm1/Blimp-1 is a master regulator of gene expression in diverse tissues of the developing embryo and adult organism. Its C-terminal zinc finger domain mediates nuclear import, DNA binding, and recruitment of the corepressors G9a and HDAC1/2. Alternatively spliced transcripts lacking exon 7 sequences encode a structurally divergent isoform (Blimp-1Δexon7) predicted to have distinct functions. Here we demonstrate that the short Blimp-1Δexon7 isoform lacks DNA binding activity and fails to bind G9a or HDAC1/2 but retains the ability to interact with PRMT5. To investigate functional roles of alternative splicing in vivo, we engineered novel mouse strains via embryonic stem (ES) cell technology. Like null mutants, embryos carrying a targeted deletion of exon 7 and exclusively expressing Blimp-1Δexon7 die at around embryonic day 10.5 (E10.5) due to placental defects. In heterozygous Δexon7 mice, there is no evidence of dominant-negative effects. Mice carrying a knock-in allele with an exon 6-exon 7 fusion express full-length Blimp-1 only, develop normally, are healthy and fertile as adults, and efficiently generate mature plasma cells. These findings strongly suggest that the short Blimp-1Δexon7 isoform is dispensable. We propose that developmentally regulated alternative splicing is influenced by chromatin structure at the locus and fine-tunes Blimp-1's functional capabilities.
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