ArticleProceedings of the National Academy of Sciences of the United States of America2012
Comparison of the H+/ATP ratios of the H+-ATP synthases from yeast and from chloroplast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 75 citations in OpenAlex.
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- Engineering of ATP synthase for enhancement of proton-to-ATP ratio.Nature communications · 2025Article
- ATP synthesis of Enterococcus hirae V-ATPase driven by sodium motive force.The Journal of biological chemistry · 2025Article
- The Warburg Effect is the result of faster ATP production by glycolysis than respiration.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Characterising the metabolic rewiring of extremely slow growing Komagataella phaffii.Microbial biotechnology · 2024Article
- Kinetic Mathematical Modeling of Oxidative Phosphorylation in Cardiomyocyte Mitochondria.Cells · 2022Review
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- Laboratory evolution of synthetic electron transport system variants reveals a larger metabolic respiratory system and its plasticity.Nature communications · 2022Article
- Insights from an information thermodynamics analysis of a synthetic molecular motor.Nature chemistry · 2022Article
- Modulation of the HFrontiers in molecular biosciences · 2022Review
- Proton motive force in plant photosynthesis dominated by ΔpH in both low and high light.Plant physiology · 2021Article
- Evolution of a biochemical model of steady-state photosynthesis.Plant, cell & environment · 2021Review
- The role of Cytochrome bPhotosynthesis research · 2021Article
- Metabolic energy conservation for fermentative product formation.Microbial biotechnology · 2021Review
- Architecture of bacterial respiratory chains.Nature reviews. Microbiology · 2021Review
- Recording and Simulating Proton-Related Metabolism in Bacterial Cell Suspensions.Frontiers in microbiology · 2021Article
- Thermodynamic efficiency, reversibility, and degree of coupling in energy conservation by the mitochondrial respiratory chain.Communications biology · 2020Article
- HPlant physiology · 2020Article
- Kinetic coupling of the respiratory chain with ATP synthase, but not proton gradients, drives ATP production in cristae membranes.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Horizontal Cell Feedback to Cone Photoreceptors in Mammalian Retina: Novel Insights From the GABA-pH Hybrid Model.Frontiers in cellular neuroscience · 2020Review
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
F(0)F(1)-ATP synthases use the free energy derived from a transmembrane proton transport to synthesize ATP from ADP and inorganic phosphate. The number of protons translocated per ATP (H(+)/ATP ratio) is an important parameter for the mechanism of the enzyme and for energy transduction in cells. Current models of rotational catalysis predict that the H(+)/ATP ratio is identical to the stoichiometric ratio of c-subunits to β-subunits. We measured in parallel the H(+)/ATP ratios at equilibrium of purified F(0)F(1)s from yeast mitochondria (c/β = 3.3) and from spinach chloroplasts (c/β = 4.7). The isolated enzymes were reconstituted into liposomes and, after energization of the proteoliposomes with acid-base transitions, the initial rates of ATP synthesis and hydrolysis were measured as a function of ΔpH. The equilibrium ΔpH was obtained by interpolation, and from its dependency on the stoichiometric ratio, [ATP]/([ADP]·[P(i)]), finally the thermodynamic H(+)/ATP ratios were obtained: 2.9 ± 0.2 for the mitochondrial enzyme and 3.9 ± 0.3 for the chloroplast enzyme. The data show that the thermodynamic H(+)/ATP ratio depends on the stoichiometry of the c-subunit, although it is not identical to the c/β ratio.
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