ArticleProceedings of the National Academy of Sciences of the United States of America2012
Rapid hybridization of nucleic acids using isotachophoresis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- A Novel Contactless Scanning Conductivity-Detection Approach for Moving Reaction Boundary Analysis in Electrophoresis Titration Sensors.Sensors (Basel, Switzerland) · 2026Article
- Microfluidic networks using isotachophoresis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Detection of EGFR mutations at pM concentration in ten minutes using a microfluidic concentration and separation module.Biomedical microdevices · 2025Article
- A method to measure molecular hybridization.PloS one · 2024Article
- Micro-/nanoscale robotics for chemical and biological sensing.Lab on a chip · 2023Review
- CRISPR Assays for Disease Diagnosis: Progress to and Barriers Remaining for Clinical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Graphene Oxide-Functionalized Thread-Based Electrofluidic Approach for DNA Hybridization.ACS omega · 2023Article
- Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases.Diagnostics (Basel, Switzerland) · 2022Review
- Isotachophoresis: Theory and Microfluidic Applications.Chemical reviews · 2022Review
- Hybridization-based DNA biosensing with a limit of detection of 4 fM in 30 s using an electrohydrodynamic concentration module fabricated by grayscale lithography.Biomicrofluidics · 2022Article
- Digital immunoassay for biomarker concentration quantification using solid-state nanopores.Nature communications · 2021Article
- Electric field-driven microfluidics for rapid CRISPR-based diagnostics and its application to detection of SARS-CoV-2.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Rapid and multi-cycle smFISH enabled by microfluidic ion concentration polarization forBiomicrofluidics · 2019Article
- Article
- Towards a Bioelectronic Computer: A Theoretical Study of a Multi-Layer Biomolecular Computing System That Can Process Electronic Inputs.International journal of molecular sciences · 2018Article
- Addressable configurations of DNA nanostructures for rewritable memory.Nucleic acids research · 2017Article
- Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Low-voltage paper isotachophoresis device for DNA focusing.Lab on a chip · 2015Article
- Nanomaterial-based electrochemical sensing of neurological drugs and neurotransmitters.Mikrochimica acta · 2015Review
- Coupling isotachophoresis with affinity chromatography for rapid and selective purification with high column utilization, part 1: theory.Analytical chemistry · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We use isotachophoresis (ITP) to control and increase the rate of nucleic acid hybridization reactions in free solution. We present a new physical model, validation experiments, and demonstrations of this assay. We studied the coupled physicochemical processes of preconcentration, mixing, and chemical reaction kinetics under ITP. Our experimentally validated model enables a closed form solution for ITP-aided reaction kinetics, and reveals a new characteristic time scale which correctly predicts order 10,000-fold speed-up of chemical reaction rate for order 100 pM reactants, and greater enhancement at lower concentrations. At 500 pM concentration, we measured a reaction time which is 14,000-fold lower than that predicted for standard second-order hybridization. The model and method are generally applicable to acceleration of reactions involving nucleic acids, and may be applicable to a wide range of reactions involving ionic reactants.
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Registered trials
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