ArticleProceedings of the National Academy of Sciences of the United States of America2012
Aligned hemozoin crystals in curved clusters in malarial red blood cells revealed by nanoprobe X-ray Fe fluorescence and diffraction.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Serum lipids and lipoproteins in malaria--a systematic review and meta-analysis.Malaria journal · 2013Pooled it
- Chemical propulsion of hemozoin crystal motion in malaria parasites.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cryo-tomography and 3D Electron Diffraction Reveal the Polar Habit and Chiral Structure of the Malaria Pigment Crystal Hemozoin.ACS central science · 2024Article
- Magneto-optical assessment of Plasmodium parasite growth via hemozoin crystal size.Scientific reports · 2024Article
- Biodereplication of Antiplasmodial Extracts: Application of the Amazonian Medicinal PlantMolecules (Basel, Switzerland) · 2022Article
- ClusterAlign: A fiducial tracking and tilt series alignment tool for thick sample tomography.Biological imaging · 2022Article
- Malaria Pigment Crystals: The Achilles' Heel of the Malaria Parasite.ChemMedChem · 2021Review
- Heme crystallization in a Chagas disease vector acts as a redox-protective mechanism to allow insect reproduction and parasite infection.PLoS neglected tropical diseases · 2018Article
- Unraveling heme detoxification in the malaria parasite by in situ correlative X-ray fluorescence microscopy and soft X-ray tomography.Scientific reports · 2017Article
- Quinolines block every step of malaria heme crystal growth.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Antimalarials inhibit hematin crystallization by unique drug-surface site interactions.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Detergent-Mediated Formation of β-Hematin: Heme Crystallization Promoted by Detergents Implicates Nanostructure Formation for Use as a Biological Mimic.Crystal growth & design · 2016Article
- Mechanisms of hematin crystallization and inhibition by the antimalarial drug chloroquine.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Identification of β-hematin inhibitors in a high-throughput screening effort reveals scaffolds with in vitro antimalarial activity.International journal for parasitology. Drugs and drug resistance · 2014Article
- Growth of Large Hematin Crystals in Biomimetic Solutions.Crystal growth & design · 2014Article
- Article
- Synthetic Hemozoin (β-Hematin) Crystals Nucleate at the Surface of Neutral Lipid Droplets that Control Their Sizes.Crystal growth & design · 2013Article
- The single crystal X-ray structure of β-hematin DMSO solvate grown in the presence of chloroquine, a β-hematin growth-rate inhibitor.Journal of the American Chemical Society · 2013Article
- Phospholipid membrane-mediated hemozoin formation: the effects of physical properties and evidence of membrane surrounding hemozoin.PloS one · 2013Article
- Malaria pigment crystals as magnetic micro-rotors: key for high-sensitivity diagnosis.Scientific reports · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human malaria parasite Plasmodium falciparum detoxifies the heme byproduct of hemoglobin digestion in infected red blood cells by sequestration into submicron-sized hemozoin crystals. The crystal is composed of heme units interlinked to form cyclic dimers via reciprocal Fe─O (propionate) bonds. Templated hemozoin nucleation was envisaged to explain a classic observation by electron microscopy of a cluster of aligned hemozoin crystals within the parasite digestive vacuole. This dovetails with evidence that acylglycerol lipids are involved in hemozoin nucleation in vivo, and nucleation of β-hematin, the synthetic analogue of hemozoin, was consistently induced at an acylglycerol-water interface via their {100} crystal faces. In order to ascertain the nature of hemozoin nucleation in vivo, we probed the mutual orientations of hemozoin crystals in situ within RBCs using synchrotron-based X-ray nanoprobe Fe fluorescence and diffraction. The X-ray patterns indicated the presence of hemozoin clusters, each comprising several crystals aligned along their needle c axes and exposing {100} side faces to an approximately cylindrical surface, suggestive of nucleation via a common lipid layer. This experimental finding, and the associated nucleation model, are difficult to reconcile with recent reports of hemozoin formation within lipid droplets in the digestive vacuole. The diffraction results are verified by a study of the nucleation process using emerging tools of three-dimensional cellular microscopy, described in the companion paper.
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