ArticleProceedings of the National Academy of Sciences of the United States of America2012
DNA sensing by electrocatalysis with hemoglobin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 36 citations in OpenAlex.
- Normalized Multipotential Redox Coding of DNA Bases for Determination of Total Nucleotide Composition.Analytical chemistry · 2023Article
- Review
- Switchable DNA wire: deposition-stripping of copper nanoclusters as an "ON-OFF" nanoswitch.Scientific reports · 2016Article
- Electrocatalysis in DNA Sensors.Polyhedron · 2014Article
- Target-responsive DNA-capped nanocontainer used for fabricating universal detector and performing logic operations.Nucleic acids research · 2014Article
- Fabrication of magneto-controlled moveable architecture to develop reusable electrochemical biosensors.Scientific reports · 2014Article
- Intraduplex DNA-mediated electrochemistry of covalently tethered redox-active reporters.Journal of the American Chemical Society · 2013Article
- Multiplexed electrochemistry of DNA-bound metalloproteins.Journal of the American Chemical Society · 2013Article
- Electrochemistry.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Electrocatalysis offers a means of electrochemical signal amplification, yet in DNA-based sensors, electrocatalysis has required high-density DNA films and strict assembly and passivation conditions. Here, we describe the use of hemoglobin as a robust and effective electron sink for electrocatalysis in DNA sensing on low-density DNA films. Protein shielding of the heme redox center minimizes direct reduction at the electrode surface and permits assays on low-density DNA films. Electrocatalysis with methylene blue that is covalently tethered to the DNA by a flexible alkyl chain linkage allows for efficient interactions with both the base stack and hemoglobin. Consistent suppression of the redox signal upon incorporation of a single cytosine-adenine (CA) mismatch in the DNA oligomer demonstrates that both the unamplified and the electrocatalytically amplified redox signals are generated through DNA-mediated charge transport. Electrocatalysis with hemoglobin is robust: It is stable to pH and temperature variations. The utility and applicability of electrocatalysis with hemoglobin is demonstrated through restriction enzyme detection, and an enhancement in sensitivity permits femtomole DNA sampling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.