ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2012
Identification and characterization of the soybean IPK1 ortholog of a low phytic acid mutant reveals an exon-excluding splice-site mutation.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 89 citations in OpenAlex.
- OsIPK1 frameshift mutations disturb phosphorus homeostasis and impair starch synthesis during grain filling in rice.Plant molecular biology · 2024Article
- Genome editing toward biofortified soybean with minimal trade-off between low phytic acid and yield.aBIOTECH · 2024Article
- An Overview of Targeted Genome Editing Strategies for Reducing the Biosynthesis of Phytic Acid: an Anti-nutrient in Crop Plants.Molecular biotechnology · 2024Review
- Alternative Splicing Variation: Accessing and Exploiting in Crop Improvement Programs.International journal of molecular sciences · 2023Review
- Mutation ofInternational journal of molecular sciences · 2022Article
- Reference Genes and Expression Analysis of Seed Desaturases Genes in Soybean Mutant Accessions.Biochemical genetics · 2022Article
- A genetic screen in Arabidopsis reveals the identical roles for RBP45d and PRP39a in 5' cryptic splice site selection.Frontiers in plant science · 2022Article
- Transcriptomic and Metabolomic Analyses Reveal a Potential Mechanism to Improve Soybean Resistance to Anthracnose.Frontiers in plant science · 2022Article
- Two efficient CRISPR/Cas9 systems for gene editing in soybean.Transgenic research · 2021Article
- Transcriptome analysis identifies differentially expressed genes in the progenies of a cross between two low phytic acid soybean mutants.Scientific reports · 2021Article
- Characterization and fine mapping of a new dwarf mutant in Brassica napus.BMC plant biology · 2021Article
- Detecting Genetic Mobility Using a Transposon-Based Marker System in Gamma-Ray Irradiated Soybean Mutants.Plants (Basel, Switzerland) · 2021Article
- AnPlants (Basel, Switzerland) · 2020Article
- Article
- A single-base change at a splice site in Wx-A1 caused incorrect RNA splicing and gene inactivation in a wheat EMS mutant line.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (Frontiers in plant science · 2018Article
- Whole genome-wide transcript profiling to identify differentially expressed genes associated with seed field emergence in two soybean low phytate mutants.BMC plant biology · 2017Article
- Common Bean Fe Biofortification Using Model Species' Lessons.Frontiers in plant science · 2017Article
- Seed Biofortification and Phytic Acid Reduction: A Conflict of Interest for the Plant?Plants (Basel, Switzerland) · 2015Review
- Seed-specific silencing of OsMRP5 reduces seed phytic acid and weight in rice.Transgenic research · 2014Article
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8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytic acid (myo-inositol 1, 2, 3, 4, 5, 6 hexakisphosphate) is an important constituent of soybean meal. Since phytic acid and its mineral salts (phytates) are almost indigestible for monogastrics, their abundance in grain food/feed causes nutritional and environmental problems; interest in breeding low phytic acid has therefore increased considerably. Based on gene mapping and the characteristics of inositol polyphosphates profile in the seeds of a soybean mutant line Gm-lpa-ZC-2, the soybean ortholog of inositol 1,3,4,5,6 pentakisphosphate (InsP(5)) 2-kinase (IPK1), which transforms InsP(5) into phytic acid, was first hypothesized as the candidate gene responsible for the low phytic acid alteration in Gm-lpa-ZC-2. One IPK1 ortholog (Glyma14g07880, GmIPK1) was then identified in the mapped region on chromosome 14. Sequencing revealed a G → A point mutation in the genomic DNA sequence and the exclusion of the entire fifth exon in the cDNA sequence of GmIPK1 in Gm-lpa-ZC-2 compared with its wild-type progenitor Zhechun No. 3. The excluded exon encodes 37 amino acids that spread across two conserved IPK1 motifs. Furthermore, complete co-segregation of low phytic acid phenotype with the G → A mutation was observed in the F(2) population of ZC-lpa x Zhexiandou No. 4 (a wild-type cultivar). Put together, the G → A point mutation affected the pre-mRNA splicing and resulted in the exclusion of the fifth exon of GmIPK1 which is expected to disrupt the GmIPK1 functionality, leading to low phytic acid level in Gm-lpa-ZC-2. Gm-lpa-ZC-2, would be a good germplasm source in low phytic acid soybean breeding.
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