ArticlePurinergic signalling2013
ATP induces the death of developing avian retinal neurons in culture via activation of P2X7 and glutamate receptors.
Article in Purinergic signalling, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- NADPH acts as an endogenous P2X7 receptor modulator to gate neuroinflammatory responses of microglia.Acta pharmacologica Sinica · 2026Article
- Cannabinoids Activate Endoplasmic Reticulum Stress Response and Promote the Death of Avian Retinal Müller Cells in Culture.Brain sciences · 2025Article
- Cell cycle regulation by ADP and IGF-1 in cultured late developing glia progenitors of the avian retina.Purinergic signalling · 2025Article
- PAV-05 Naphthoquinone Potently Inhibit Zika Virus Replication in Infected Cells.Current topics in medicinal chemistry · 2025Article
- The Healthy and Diseased Retina Seen through Neuron-Glia Interactions.International journal of molecular sciences · 2024Review
- Purinergic Receptors P2X7 and P2X4 as Markers of Disease Progression in the rd10 Mouse Model of Inherited Retinal Dystrophy.International journal of molecular sciences · 2022Article
- Purinergic signaling in the retina: From development to disease.Brain research bulletin · 2019Review
- Cannabinoids Induce Cell Death and Promote P2X7 Receptor Signaling in Retinal Glial Progenitors in Culture.Molecular neurobiology · 2019Article
- Inhibition by Marine Algae of Chikungunya Virus Isolated From Patients in a Recent Disease Outbreak in Rio de Janeiro.Frontiers in microbiology · 2019Article
- P2X receptor overexpression induced by soluble oligomers of amyloid beta peptide potentiates synaptic failure and neuronal dyshomeostasis in cellular models of Alzheimer's disease.Neuropharmacology · 2018Article
- Adenine Nucleotides Control Proliferation In Vivo of Rat Retinal Progenitors by P2YMolecular neurobiology · 2017Article
- Neuronal P2X7 Receptor: Involvement in Neuronal Physiology and Pathology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Review
- P2X7 receptor large pore signaling in avian Müller glial cells.Journal of bioenergetics and biomembranes · 2017Article
- Glutathione induces GABA release through P2XNeurogenesis (Austin, Tex.) · 2017Article
- Extracellular ATP protects pancreatic duct epithelial cells from alcohol-induced damage through P2Y1 receptor-cAMP signal pathway.Cell biology and toxicology · 2016Article
- Article
- Potentiation of temozolomide antitumor effect by purine receptor ligands able to restrain the in vitro growth of human glioblastoma stem cells.Purinergic signalling · 2015Article
- The nitric oxide-cGKII system relays death and survival signals during embryonic retinal development via AKT-induced CREB1 activation.Cell death and differentiation · 2014Article
- Role of P2X7 receptors in neuronal death in the retina.Neural regeneration research · 2014Article
- GABA and Glutamate Uptake and Metabolism in Retinal Glial (Müller) Cells.Frontiers in endocrinology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous data suggest that nucleotides are important mitogens in the developing retina. Here, the effect of ATP on the death of cultured chick embryo retina cells was investigated. In cultures obtained from retinas of 7-day-old chick embryos (E7) that were cultivated for 2 days (E7C2), both ATP and BzATP induced a ∼30 % decrease in cell viability that was time- and dose-dependent and that could be blocked by 0.2 mM oxidized ATP or 0.3 μM KN-62. An increase in cleaved caspase-3 levels and in the number of TUNEL-positive cells was observed when cultures were incubated with 3 mM ATP and immunolabeling for cleaved-caspase 3 was observed over neurons but not over glial cells. ATP-dependent cell death was developmentally regulated, the maximal levels being detected by E7C2-3. Nucleotides were able to increase neuronal ethidium bromide and sulforhodamine B uptake in mixed and purified neuronal cultures, an effect that was blocked by the antagonists Brilliant Blue G and oxidized ATP. In contrast, nucleotide-induced cell death was observed only in mixed cultures, but not in purified cultures of neurons or glia. ATP-induced neuronal death was blocked by the glutamatergic antagonists MK801 and DNQX and activation of P2X7 receptors by ATP decreased the uptake of [(3)H]-D-aspartate by cultured glial cells with a concomitant accumulation of it in the extracellular medium. These results suggest that ATP induces apoptosis of chick embryo retinal neurons in culture through activation of P2X7 and glutamate ionotropic receptors. Involvement of a P2X7 receptor-mediated inhibition of the glial uptake of glutamate is suggested.
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