ArticleJournal of industrial microbiology & biotechnology2012
Effect of transport proteins on L-isoleucine production with the L-isoleucine-producing strain Corynebacterium glutamicum YILW.
Article in Journal of industrial microbiology & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 52 citations in OpenAlex.
- Metabolic engineering strategies for sustainable de novo isobutylamine production by Corynebacterium glutamicum.Biotechnology for biofuels and bioproducts · 2026Article
- Precise l-threonine-to-l-isoleucine pathway regulation for engineering high-efficiency whole-cell biocatalysts.Synthetic and systems biotechnology · 2026Article
- Harnessing prokaryotic amino acid transporters for metabolic engineering: mechanisms and biotechnological applications.Synthetic and systems biotechnology · 2026Review
- Deciphering a missing piece of the branched-chain amino acids uptake puzzle: YhjE is an L-isoleucine and L-valine transporter inFrontiers in microbiology · 2025Article
- Branched-chain amino acids: physico-chemical properties, industrial synthesis and role in signaling, metabolism and energy production.Amino acids · 2024Review
- Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.Molecules (Basel, Switzerland) · 2024Review
- Tryptophan-Based Hyperproduction of Bioindigo by Combinatorial Overexpression of Two Different Tryptophan Transporters.Journal of microbiology and biotechnology · 2024Article
- Metabolic engineering of Corynebacterium glutamicum for producing branched chain amino acids.Microbial cell factories · 2021Review
- Microbial methionine transporters and biotechnological applications.Applied microbiology and biotechnology · 2021Review
- Systematic engineering of branch chain amino acid supply modules for the enhanced production of bacitracin fromMetabolic engineering communications · 2020Article
- Real-Time Optical Detection of Isoleucine in Living Cells through a Genetically-Encoded Nanosensor.Sensors (Basel, Switzerland) · 2019Article
- Enhancement of precursor amino acid supplies for improving bacitracin production by activation of branched chain amino acid transporter BrnQ and deletion of its regulator geneSynthetic and systems biotechnology · 2018Article
- Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-produced with L-isoleucine in Corynebacterium glutamicum WM001.Microbial cell factories · 2018Article
- Systems metabolic engineering strategies for the production of amino acids.Synthetic and systems biotechnology · 2017Review
- Optimization of carbon source and glucose feeding strategy for improvement of L-isoleucine production byBiotechnology, biotechnological equipment · 2015Article
- Production of 2-ketoisocaproate with Corynebacterium glutamicum strains devoid of plasmids and heterologous genes.Microbial biotechnology · 2015Article
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5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies have shown that the deletion of brnQ from the Corynebacterium glutamicum chromosome results in a significant reduction in L-isoleucine uptake rates, while overexpression of brnFE leads to enhanced L-isoleucine export rates. Given that net excretion rates would be an important factor for high titers of L-isoleucine accumulation, we have tested the notion that decreased L-isoleucine uptake combined with increased L-isoleucine excretion will further improve high-yield strains that are currently used for the industrial-scale production of L-isoleucine. To examine the effect of the two carriers on L-isoleucine accumulation in L-isoleucine producer C. glutamicum YILW, we constructed a brnQ deletion mutant (C. glutamicum YILW∆brnQ) and two brnFE overexpressors (C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE). Compared to the original strain, the efflux rate of the brnQ mutant increased from 19.0 to 23.6 nmol min(-1) mg (dry wt)(-1) and its L-isoleucine titer increased from 154.3 mM (20.2 g l(-1)) to 170.3 mM (22.3 g l(-1)). The efflux rates of C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE were 33.5 and 39.1 nmol min(-1) mg (dry wt)(-1), and their L-isoleucine production titers were 197.2 mM (25.9 g l(-1)) and 221.0 mM (29.0 g l(-1)), respectively. Our results suggest that modifications of the transport system could provide a promising avenue for further increasing L-isoleucine yield in the L-isoleucine producer.
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