ArticleIntegrative biology : quantitative biosciences from nano to macro2012
Differential effects of a soluble or immobilized VEGFR-binding peptide.
Article in Integrative biology : quantitative biosciences from nano to macro, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
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- Thiol-norbornene photo-click hydrogels for tissue engineering applications.Journal of applied polymer science · 2015Article
- Highly angiogenic peptide nanofibers.ACS nano · 2015Article
- Peptide-modified zwitterionic porous hydrogels for endothelial cell and vascular engineering.BioResearch open access · 2014Article
- Avidity-controlled delivery of angiogenic peptides from injectable molecular-recognition hydrogels.Tissue engineering. Part A · 2014Article
- Biomaterial arrays with defined adhesion ligand densities and matrix stiffness identify distinct phenotypes for tumorigenic and nontumorigenic human mesenchymal cell types.Biomaterials science · 2014Article
- One-pot Synthesis of Elastin-like Polypeptide Hydrogels with Grafted VEGF-Mimetic Peptides.Biomaterials science · 2014Article
- Differential effects of cell adhesion, modulus and VEGFR-2 inhibition on capillary network formation in synthetic hydrogel arrays.Biomaterials · 2014Article
- Context clues: the importance of stem cell-material interactions.ACS chemical biology · 2014Review
- Specific VEGF sequestering to biomaterials: influence of serum stability.Acta biomaterialia · 2013Article
- ECM-incorporated hydrogels cross-linked via native chemical ligation to engineer stem cell microenvironments.Biomacromolecules · 2013Article
- Patterned self-assembled monolayers: efficient, chemically defined tools for cell biology.Chembiochem : a European journal of chemical biology · 2012Review
- Combinatorial screening of chemically defined human mesenchymal stem cell culture substrates.Journal of materials chemistry · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Regulating endothelial cell behavior is a key step in understanding and controlling neovascularization for both pro-angiogenic and anti-angiogenic therapeutic strategies. Here, we characterized the effects of a covalently immobilized peptide mimic of vascular endothelial growth factor, herein referred to as VEGF receptor-binding peptide (VR-BP), on human umbilical vein endothelial cell (HUVEC) behavior. Self-assembled monolayer arrays presenting varied densities of covalently immobilized VR-BP and varied densities of the fibronectin-derived cell adhesion peptide Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP) were used to probe for changes in HUVEC attachment, proliferation and tubulogenesis. In a soluble form, VR-BP exhibited pro-angiogenic effects in agreement with previous studies, indicated by increases in HUVEC proliferation. However, when presented to cells in an insoluble context, covalently immobilized VR-BP inhibited several pro-angiogenic HUVEC behaviors, including attachment and proliferation, and also inhibited HUVEC response to soluble recombinant VEGF protein. Furthermore, substrates with covalently immobilized VR-BP also modulated HUVEC tubulogenesis when a matrigel overlay assay was used to provide cells with a pseudo-three dimensional environment. Taken together, these results demonstrate that the context in which ligands are presented to cell surface receptors strongly influences their effects, and that the same ligand can be an agonist or an antagonist depending on the manner of presentation to the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.