ArticleAntimicrobial agents and chemotherapy2012
Role of ISKpn7 and deletions in blaKPC gene expression.
Article in Antimicrobial agents and chemotherapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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70 citing papers in PubMed, 110 citations in OpenAlex.
- Epidemiology and molecular characterization of bacteremia due to carbapenem-resistant Klebsiella pneumoniae in transplant recipients.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2013Trial
- Carbapenem-Resistant Enterobacterales (CRE) in Europe: Surveillance Challenges, Emerging Threats and Public Health Preparedness.Antibiotics (Basel, Switzerland) · 2026Review
- Characterization of GUA-1, a chromosomally encoded ESBL fromAntimicrobial agents and chemotherapy · 2026Article
- KPC-producing Klebsiella pneumoniae in Switzerland from 2024 to 2025: comparative genomics and cefiderocol resistance mechanisms analyses.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Carbapenemases: epidemiology, detection and management in a changing global landscape.JAC-antimicrobial resistance · 2026Review
- The effect of exogenous glutathione on meropenem susceptibility in Klebsiella pneumoniae-carbapenemases (KPC)-producing bacteria.The Journal of antibiotics · 2025Article
- Molecular Characterization of Carbapenemase Genes in Carbapenem-Resistant Klebsiella pneumoniae Isolates.Current microbiology · 2025Article
- Characterization of aMicrobiology spectrum · 2025Article
- Presence of Coliforms and Reduced Water Quality in the Second Biggest Reservoir in São Paulo, Brazil.Life (Basel, Switzerland) · 2025Article
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- Molecular epidemiology of carbapenem-resistant hypervirulentFrontiers in cellular and infection microbiology · 2025Article
- Developing a framework for tracking antimicrobial resistance gene movement in a persistent environmental reservoir.npj antimicrobials and resistance · 2024Article
- Characteristics of antibiotic resistance mechanisms and genes ofOpen medicine (Warsaw, Poland) · 2023Review
- Transmission of Nonconjugative Virulence or Resistance Plasmids Mediated by a Self-Transferable IncN3 Plasmid from Carbapenem-Resistant Klebsiella pneumoniae.Microbiology spectrum · 2022Article
- How Do Transposable Elements Activate Expression of Transcriptionally Silent Antibiotic Resistance Genes?International journal of molecular sciences · 2022Review
- Genomic Features Associated with the Degree of Phenotypic Resistance to Carbapenems in Carbapenem-Resistant Klebsiella pneumoniae.mSystems · 2021Article
- Genetic Diversity of Multidrug-ResistantFrontiers in microbiology · 2021Article
- Undetectable Production of the VIM-1 Carbapenemase in anFrontiers in microbiology · 2021Article
- Clonal Spread and Intra- and Inter-Species Plasmid Dissemination Associated WithFrontiers in microbiology · 2021Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The carbapenemase-encoding bla(KPC) gene, which is rapidly spreading in Gram-negative rods, is located on a Tn3-based transposon, Tn4401, which carries a polymorphic region giving rise to five isoforms (a, b, c, d, and e) that is located immediately upstream of the bla(KPC) gene and thus likely involved in its expression. Using 5' rapid amplification of cDNA ends (5'RACE), we identified three potential promoter sequences (P1, P2, and P3) upstream of the bla(KPC) gene, of which only P1 (absent from isoforms c and d) and P2 (present in all isoforms, with a -35 box located inside the right inverted repeat of ISKpn7) were shown to be true promoters involved in expression. One representative of each different promoter combination of Tn4401, i.e., P2 alone (isoform c), P1-P2 (isoform a), and P1-P2-P3 (isoform b), was cloned into an Escherichia coli plasmid vector. Using reverse transcription-PCR (RT-PCR), the highest level of expression was obtained with isoform a (P1 and P2), which is also the most commonly encountered form in enterobacterial clinical isolates, followed by isoforms b (P1, P2, and P3) and c (P2 only). These differences in expression led to slight differences in MIC values of carbapenems. In silico analysis of the DNA sequence of isoform b revealed a stem-loop structure that is likely responsible for strong stops observed in 5'RACE experiments and for decreased expression compared to that with isoform a (P1 and P2). In addition, such structures could also be at the origin for the deletions observed in isoforms a and c. Taken together, these results indicate that the P1 and P2 promoters both contribute to the expression of the bla(KPC) gene and that the construct with the highest level of expression is that possessing isoform a, which is also the most commonly encountered form in clinical isolates.
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