ArticleHaematologica2012
Improved platelet survival after cold storage by prevention of glycoprotein Ibα clustering in lipid rafts.
Article in Haematologica, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Platelet Storage-Problems, Improvements, and New Perspectives.International journal of molecular sciences · 2024Review
- The role of platelet desialylation as a biomarker in primary immune thrombocytopenia: mechanisms and therapeutic perspectives.Frontiers in immunology · 2024Review
- Inhibition of GPIb-α-mediated apoptosis signaling enables cold storage of platelets.Haematologica · 2023Article
- Enhanced hepatic clearance of hyposialylated platelets explains thrombocytopenia in GNE-related macrothrombocytopenia.Blood advances · 2022Article
- Function of Platelet Glycosphingolipid Microdomains/Lipid Rafts.International journal of molecular sciences · 2020Review
- The role of neuraminidase 1 and 2 in glycoprotein Ibα-mediated integrin αIIbβ3 activation.Haematologica · 2020Article
- Akt-mediated platelet apoptosis and its therapeutic implications in immune thrombocytopenia.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Effect of Pneumatic Tubing System Transport on Platelet Apheresis Units.Cardiovascular engineering and technology · 2018Article
- Refrigeration-Induced Binding of von Willebrand Factor Facilitates Fast Clearance of Refrigerated Platelets.Arteriosclerosis, thrombosis, and vascular biology · 2017Article
- Protein kinase A determines platelet life span and survival by regulating apoptosis.The Journal of clinical investigation · 2017Article
- Recent developments in ex vivo platelet production.Cytotechnology · 2016Review
- Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro.Journal of visualized experiments : JoVE · 2016Article
- Desialylation is a mechanism of Fc-independent platelet clearance and a therapeutic target in immune thrombocytopenia.Nature communications · 2015Article
- Syk and Src family kinases regulate C-type lectin receptor 2 (CLEC-2)-mediated clustering of podoplanin and platelet adhesion to lymphatic endothelial cells.The Journal of biological chemistry · 2014Article
- Hemostatic function of apheresis platelets stored at 4°C and 22°C.Shock (Augusta, Ga.) · 2014Article
- Platelet interaction with von Willebrand factor is enhanced by shear-induced clustering of glycoprotein Ibα.Haematologica · 2013Article
- Patient autoantibodies induce platelet destruction signals via raft-associated glycoprotein Ibα and Fc RIIa in immune thrombocytopenia.Haematologica · 2013Article
- State of the art in platelet function testing.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2013Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStoring platelets for transfusion at room temperature increases the risk of microbial infection and decreases platelet functionality, leading to out-date discard rates of up to 20%. Cold storage may be a better alternative, but this treatment leads to rapid platelet clearance after transfusion, initiated by changes in glycoprotein Ibα, the receptor for von Willebrand factor. DESIGN AND
methodsWe examined the change in glycoprotein Ibα distribution using Förster resonance energy transfer by time-gated fluorescence lifetime imaging microscopy.
resultsCold storage induced deglycosylation of glycoprotein Ibα ectodomain, exposing N-acetyl-D-glucosamine residues, which sequestered with GM1 gangliosides in lipid rafts. Raft-associated glycoprotein Ibα formed clusters upon binding of 14-3-3ζ adaptor proteins to its cytoplasmic tail, a process accompanied by mitochondrial injury and phosphatidyl serine exposure. Cold storage left glycoprotein Ibα surface expression unchanged and although glycoprotein V decreased, the fall did not affect glycoprotein Ibα clustering. Prevention of glycoprotein Ibα clustering by blockade of deglycosylation and 14-3-3ζ translocation increased the survival of cold-stored platelets to above the levels of platelets stored at room temperature without compromising hemostatic functions.
conclusionsWe conclude that glycoprotein Ibα translocates to lipid rafts upon cold-induced deglycosylation and forms clusters by associating with 14-3-3ζ. Interference with these steps provides a means to enable cold storage of platelet concentrates in the near future.
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