ArticleJournal of immunological methods2012
Enzyme-linked immunosorbent assay (ELISA) and blocking with bovine serum albumin (BSA)--not all BSAs are alike.
Article in Journal of immunological methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed, 178 citations in OpenAlex.
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- Development and Validation of an Enzyme-Linked Immunosorbent Assay for Measuring Factor XI in Intravenous Immunoglobulin Products by Mitigating Heterophilic Antibody Interference.Journal of microbiology and biotechnology · 2026Article
- Nanoporous Gold Nanoparticles-Modified Electrode for the Detection of Endotoxins.Micromachines · 2025Article
- Development of an In-House ELISA for Serological Detection of Equine Herpesvirus-1/4 Antibodies in Turkish Horses.Animals : an open access journal from MDPI · 2025Article
- Establishment of a Rapid and Convenient Fluoroimmunoassay Platform Using Antibodies Against PDL1 and HER2.Current issues in molecular biology · 2025Article
- Recent advances in rapid detection of Helicobacter pylori by lateral flow assay.Archives of microbiology · 2025Review
- Development of ELISA Assay for Antibody Detection in Cancer Vaccine Research.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Sensing Platform Based on Gold Nanoclusters and Nanoporous Anodic Alumina for Preeclampsia Detection.Biosensors · 2024Article
- Adsorption and Morphology Analysis of Bovine Serum Albumin on a Micropillar-Enhanced Quartz Crystal Microbalance.The journal of physical chemistry. B · 2024Article
- Development and characterization of monoclonal antibodies recognizing nucleocapsid protein of multiple SARS-CoV-2 variants.Heliyon · 2024Article
- High-Throughput Bioassay for Detection of Latent Fungi in Postharvest Produce.Applied biochemistry and biotechnology · 2024Article
- Membrane-Free Lateral Flow Assay with the Active Control of Fluid Transport for Ultrasensitive Cardiac Biomarker Detection.Analytical chemistry · 2024Article
- Medical Relevance, State-of-the-Art and Perspectives of "Sweet Metacode" in Liquid Biopsy Approaches.Diagnostics (Basel, Switzerland) · 2024Review
- Highly sensitive therapeutic drug monitoring of infliximab in serum by targeted mass spectrometry in comparison to ELISA data.Clinical proteomics · 2024Article
- Sensing antibody functions with a novel CCR8-responsive engineered cell.Acta biochimica Polonica · 2024Article
- Simplified PCR-Based Quantification of Proteins with DNA Aptamers and Methylcellulose as a Blocking Agent.International journal of molecular sciences · 2023Article
- Article
- Blocking Nonspecific Interactions Using Y-Shape Poly(ethylene glycol).International journal of molecular sciences · 2023Article
- Mass Spectrometric Identification of BSA Covalently Captured onto a Chip for Atomic Force Microscopy.International journal of molecular sciences · 2023Article
- A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch-Agarose Mixture and Its Application for Convenient Protein-Protein Interaction Analysis.Methods and protocols · 2023Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter well. While studying the interactions of the vaccinia virus complement control protein (VCP) with complement, we found non-specific binding of VCP to BSA and identify a BSA preparation that did not result in non-specific binding. This work draws attention to the fact that not all BSA preparations are alike. It also highlights the need to perform critical controls to ensure that ELISA reactants do not inappropriately bind to the blocking agent.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.