ArticleNano letters2012
Proteolytic activity at quantum dot-conjugates: kinetic analysis reveals enhanced enzyme activity and localized interfacial "hopping".
Article in Nano letters, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- AND-gated protease-activated nanosensors for programmable detection of anti-tumour immunity.Nature nanotechnology · 2025Article
- Immobilization of Thiol-Modified Horseradish Peroxidase on Gold Nanoparticles Enhances Enzyme Stability and Prevents Proteolytic Digestion.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Green nanobiocatalysts: enhancing enzyme immobilization for industrial and biomedical applications.PeerJ · 2024Review
- Article
- Hybrid Nucleic Acid-Quantum Dot Assemblies as Multiplexed Reporter Platforms for Cell-Free Transcription Translation-Based Biosensors.ACS synthetic biology · 2022Article
- Spacer Matters: All-Peptide-Based Ligand for Promoting Interfacial Proteolysis and Plasmonic Coupling.Nano letters · 2022Article
- Determining the Cytosolic Stability of Small DNA NanostructuresNano letters · 2022Article
- Implementing Multi-Enzyme Biocatalytic Systems Using Nanoparticle Scaffolds.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Misuse of the Michaelis-Menten rate law for protein interaction networks and its remedy.PLoS computational biology · 2020Review
- Sensing with photoluminescent semiconductor quantum dots.Methods and applications in fluorescence · 2019Article
- Allosteric inhibition of α-thrombin enzymatic activity with ultrasmall gold nanoparticles.Nanoscale advances · 2019Article
- Beyond the Michaelis-Menten equation: Accurate and efficient estimation of enzyme kinetic parameters.Scientific reports · 2017Article
- Engineering Immunological Tolerance Using Quantum Dots to Tune the Density of Self-Antigen Display.Advanced functional materials · 2017Article
- Label-Free Fluorescent Detection of Trypsin Activity Based on DNA-Stabilized Silver Nanocluster-Peptide Conjugates.Sensors (Basel, Switzerland) · 2016Article
- Polyvalent Catalysts Operating on Polyvalent Substrates: A Model for Surface-Controlled Reactivity.Angewandte Chemie (International ed. in English) · 2016Article
- 3,4-Dihydroxyphenylalanine Peptides as Nonperturbative Quantum Dot Sensors of Aminopeptidase.ACS nano · 2016Article
- Quantum Dot-Modified Paper-Based Assay for Glucose Screening.Mikrochimica acta · 2016Article
- QD-Based FRET Probes at a Glance.Sensors (Basel, Switzerland) · 2015Review
- Sensitive and selective plasmon ruler nanosensors for monitoring the apoptotic drug response in leukemia.ACS nano · 2014Article
- DNA-based sensor for real-time measurement of the enzymatic activity of human topoisomerase I.Sensors (Basel, Switzerland) · 2013Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Recent studies show that polyvalent, ligand-modified nanoparticles provide significantly enhanced binding characteristics compared to isolated ligands. Here, we assess the ability of substrate-modified nanoparticles to provide enhanced enzymatic activity. Energy transfer assays allowed quantitative, real-time measurement of proteolytic digestion at polyvalent quantum dot-peptide conjugates. Enzymatic progress curves were analyzed using an integrated Michaelis-Menten (MM) formalism, revealing mechanistic details, including deviations from classic MM-behavior. A "hopping" mode of proteolysis at the nanoparticle was identified, confirming enhanced activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.