ArticleThe Journal of biological chemistry2012
Mitochondrial cysteine synthase complex regulates O-acetylserine biosynthesis in plants.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 69 citations in OpenAlex.
- Sulfur metabolism-dependent retrograde signaling for oxidative stress acclimation.Journal of experimental botany · 2026Review
- Sulfur in dialogue with phosphorus, nitrogen, and iron: regulatory networks in plant nutrient homeostasis.Journal of experimental botany · 2026Review
- Sulfur Homeostasis in Rice as a Dynamic Regulatory Network: Functional Genomics, Metabolic Crosstalk, and miR395-Mediated Control.Molecular biotechnology · 2026Review
- Adaptive modifications in plant sulfur metabolism over evolutionary time.Journal of experimental botany · 2024Review
- Sulfur signaling and starvation response in Arabidopsis.iScience · 2022Review
- Assessing the Kinetics of Metabolite Uptake and Utilization by Isolated Mitochondria Using Selective Reaction Monitoring Mass Spectrometry (SRM-MS).Methods in molecular biology (Clifton, N.J.) · 2022Article
- Micrografting Provides Evidence for Systemic Regulation of Sulfur Metabolism between Shoot and Root.Plants (Basel, Switzerland) · 2021Article
- Interplay between hydrogen sulfide and other signaling molecules in the regulation of guard cell signaling and abiotic/biotic stress response.Plant communications · 2021Review
- A molecular switch in sulfur metabolism to reduce arsenic and enrich selenium in rice grain.Nature communications · 2021Article
- Metabolism and Regulatory Functions ofFrontiers in plant science · 2021Review
- Chloroplast-associated molecular patterns as concept for fine-tuned operational retrograde signalling.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Molecular cloning of putative chloroplastic cysteine synthase in Leucaena leucocephala.Journal of plant research · 2020Article
- Plant sulphur metabolism is stimulated by photorespiration.Communications biology · 2019Article
- The PhotorespiratoryFrontiers in plant science · 2018Article
- Sulfur availability regulates plant growth via glucose-TOR signaling.Nature communications · 2017Article
- Drought stress in maize causes differential acclimation responses of glutathione and sulfur metabolism in leaves and roots.BMC plant biology · 2016Article
- Characterization of the serine acetyltransferase gene family of Vitis vinifera uncovers differences in regulation of OAS synthesis in woody plants.Frontiers in plant science · 2015Article
- Impairment of Respiratory Chain under Nutrient Deficiency in Plants: Does it Play a Role in the Regulation of Iron and Sulfur Responsive Genes?Frontiers in plant science · 2015Article
- Transceptors at the boundary of nutrient transporters and receptors: a new role for Arabidopsis SULTR1;2 in sulfur sensing.Frontiers in plant science · 2014Article
- The significance of cysteine synthesis for acclimation to high light conditions.Frontiers in plant science · 2014Article
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
Abstract
Cysteine synthesis is catalyzed by serine acetyltransferase (SAT) and O-acetylserine (thiol) lyase (OAS-TL) in the cytosol, plastids, and mitochondria of plants. Biochemical analyses of recombinant plant SAT and OAS-TL indicate that the reversible association of the proteins in the cysteine synthase complex (CSC) controls cellular sulfur homeostasis. However, the relevance of CSC formation in each compartment for flux control of cysteine synthesis remains controversial. Here, we demonstrate the interaction between mitochondrial SAT3 and OAS-TL C in planta by FRET and establish the role of the mitochondrial CSC in the regulation of cysteine synthesis. NMR spectroscopy of isolated mitochondria from WT, serat2;2, and oastl-C plants showed the SAT-dependent export of OAS. The presence of cysteine resulted in reduced OAS export in mitochondria of oastl-C mutants but not in WT mitochondria. This is in agreement with the stronger in vitro feedback inhibition of free SAT by cysteine compared with CSC-bound SAT and explains the high OAS export rate of WT mitochondria in the presence of cysteine. The predominant role of mitochondrial OAS synthesis was validated in planta by feeding [(3)H]serine to the WT and loss-of-function mutants for OAS-TLs in the cytosol, plastids, and mitochondria. On the basis of these results, we propose a new model in which the mitochondrial CSC acts as a sensor that regulates the level of SAT activity in response to sulfur supply and cysteine demand.
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