Evidence map›Paper›PMID 22730131›Full record

ArticleJournal of bacteriology2012

Molecular characterization of the flagellar hook in Bacillus subtilis.

Colleen R Courtney, Loralyn M Cozy, Daniel B Kearns

Open access · bronzeAbstract read
In one paragraph

Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 48 citations in OpenAlex.

  1. Article
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  7. An overview of the structure and function of the flagellar hook FlgE protein.World journal of microbiology & biotechnology · 2023
    Review
  8. Article
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  10. Review
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  12. Article
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  14. Dynamic exchange of two types of stator units inComputational and structural biotechnology journal · 2020
    Review
  15. TheFrontiers in microbiology · 2020
    Article
  16. Article
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  19. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Colleen R CourtneyIndiana University, Department of Biology, Bloomington, Indiana, USA.
Loralyn M Cozy
Daniel B Kearns
Indiana University Bloomington · US

Funding

The mechanism of bistable motility gene expression in Bacillus subtilis.R01GM093030 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI KEARNS, DANIEL B · 2010 to 2018
$2.6M
NIGMS NIH HHS GM093030NIGMS NIH HHS R01 GM093030
6 · The paper itself

Abstract

The structure of the Gram-positive flagellum is poorly understood, and Bacillus subtilis encodes three proteins homologous to the flagellar hook protein from Salmonella enterica. Here we generated a modified B. subtilis hook protein that could be fluorescently stained using a cysteine-reactive dye. We used the fluorescently labeled hook to demonstrate that FlgE is the hook structural protein and that FliK regulated hook length. We further demonstrate that two proteins of unknown function, FlhO and FlhP, and the putative hook cap, FlgD, were required for hook assembly, such that when flhO, flhP, or flgD was mutated, hook protein was secreted into the supernatant. All mutants defective in hook completion resulted in homogeneously reduced σ(D)-dependent gene expression due to the action of the anti-sigma factor FlgM.

Indexed as

Bacillus subtilisBacterial ProteinsFlagellaGene Expression Regulation, BacterialPromoter Regions, GeneticSigma FactorBacterial ProteinsFlgE protein, BacteriaFlgM protein, BacteriaFliK protein, BacteriaSigma Factor

Identifiers

PMID22730131
PMCPMC3415477
OpenAlexW1989949806

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.