ArticleApplied and environmental microbiology2012
MhbT is a specific transporter for 3-hydroxybenzoate uptake by Gram-negative bacteria.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Genomic and functional insights into aromatic compound degradation byMicrobiology spectrum · 2026Article
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- Maleylpyruvic Acid-Inducible Gene Expression System and Its Application for the Development of Gentisic Acid Biosensors.Analytical chemistry · 2024Article
- MhpA Is a Hydroxylase Catalyzing the Initial Reaction of 3-(3-Hydroxyphenyl)Propionate Catabolism inApplied and environmental microbiology · 2020Article
- Engineering a Synthetic Pathway for Gentisate inFrontiers in bioengineering and biotechnology · 2020Article
- Mapping the diversity of microbial lignin catabolism: experiences from the eLignin database.Applied microbiology and biotechnology · 2019Review
- ndpT encodes a new protein involved in nicotine catabolism by Sphingomonas melonis TY.Applied microbiology and biotechnology · 2018Article
- DdvK, a Novel Major Facilitator Superfamily Transporter Essential for 5,5'-Dehydrodivanillate Uptake by Sphingobium sp. Strain SYK-6.Applied and environmental microbiology · 2018Article
- 3,6-Dichlorosalicylate Catabolism Is Initiated by the DsmABC Cytochrome P450 Monooxygenase System in Rhizorhabdus dicambivorans Ndbn-20.Applied and environmental microbiology · 2018Article
- Eukaryotic transporters for hydroxyderivatives of benzoic acid.Scientific reports · 2017Article
- Characterization of a Novel Nicotine Degradation Gene ClusterFrontiers in microbiology · 2017Article
- γ-Resorcylate catabolic-pathway genes in the soil actinomycete Rhodococcus jostii RHA1.Applied and environmental microbiology · 2015Article
- Expression, purification and reconstitution of the 4-hydroxybenzoate transporter PcaK from Acinetobacter sp. ADP1.Protein expression and purification · 2014Article
- Novel tripartite aromatic acid transporter essential for terephthalate uptake in Comamonas sp. strain E6.Applied and environmental microbiology · 2013Article
- mhpT encodes an active transporter involved in 3-(3-hydroxyphenyl)propionate catabolism by Escherichia coli K-12.Applied and environmental microbiology · 2013Article
- GenR, an IclR-type regulator, activates and represses the transcription of gen genes involved in 3-hydroxybenzoate and gentisate catabolism in Corynebacterium glutamicum.Journal of bacteriology · 2013Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Klebsiella pneumoniae M5a1 is capable of utilizing 3-hydroxybenzoate via gentisate, and the 6.3-kb gene cluster mhbRTDHIM conferred the ability to grow on 3-hydroxybenzoate to Escherichia coli and Pseudomonas putida PaW340. Four of the six genes (mhbDHIM) encode enzymes converting 3-hydroxybenzoate to pyruvate and fumarate via gentisate. MhbR is a gene activator, and MhbT is a hypothetical protein belonging to the transporter of the aromatic acid/H(+) symporter family. Since a transporter for 3-hydrxybenzoate uptake has not been characterized to date, we investigated whether MhbT is responsible for the uptake of 3-hydroxybenzoate, its metabolic intermediate gentisate, or both. The MhbT-green fluorescent protein (GFP) fusion protein was located on the cytoplasmic membrane. P. putida PaW340 containing mhbRΔTDHIM could not grow on 3-hydroxybenzoate; however, supplying mhbT in trans allowed the bacterium to grow on the substrate. K. pneumoniae M5a1 and P. putida PaW340 containing recombinant MhbT transported (14)C-labeled 3-hydroxybenzoate but not (14)C-labeled gentisate and benzoate into the cells. Site-directed mutagenesis of two conserved amino acid residues (Asp-82 and Asp-314) and a less-conserved residue (Val-311) among the members of the symporter family in the hydrophilic cytoplasmic loops resulted in the loss of 3-hydroxybenzoate uptake by P. putida PaW340 carrying the mutant proteins. Hence, we demonstrated that MhbT is a specific 3-hydroxybenzoate transporter.
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