ArticleNature cell biology2012
Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment.
Article in Nature cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 44 citations in OpenAlex.
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- Cytoplasmic control of spindle architecture.Nature cell biology · 2025Article
- Cell state-specific cytoplasmic density controls spindle architecture and scaling.Nature cell biology · 2025Article
- Versatile gamma-tubulin complexes contribute to the dynamic organization of MTOCs during Drosophila spermatogenesis.Communications biology · 2024Article
- Functional Analysis of Hydrolethalus Syndrome Protein HYLS1 in Ciliogenesis and Spermatogenesis inFrontiers in cell and developmental biology · 2020Article
- A magic bullet for targeting cancers with supernumerary centrosomes.The EMBO journal · 2019Article
- Inhibition of CPAP-tubulin interaction prevents proliferation of centrosome-amplified cancer cells.The EMBO journal · 2019Article
- Meiotic spindle assembly and chromosome segregation in oocytes.The Journal of cell biology · 2016Review
- Molecular basis for CPAP-tubulin interaction in controlling centriolar and ciliary length.Nature communications · 2016Article
- Centriolar CPAP/SAS-4 Imparts Slow Processive Microtubule Growth.Developmental cell · 2016Article
- CPAP promotes timely cilium disassembly to maintain neural progenitor pool.The EMBO journal · 2016Article
- Arl2- and Msps-dependent microtubule growth governs asymmetric division.The Journal of cell biology · 2016Article
- Regulation of the centrosome cycle.Molecular & cellular oncology · 2016Review
- Article
- Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP.eLife · 2015Article
- Article
- Centrosome function and assembly in animal cells.Nature reviews. Molecular cell biology · 2015Review
- Dissecting the function and assembly of acentriolar microtubule organizing centers in Drosophila cells in vivo.PLoS genetics · 2015Article
- The centrosome and its duplication cycle.Cold Spring Harbor perspectives in biology · 2015Review
- Amorphous no more: subdiffraction view of the pericentriolar material architecture.Trends in cell biology · 2014Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Regulated centrosome biogenesis is required for accurate cell division and for maintaining genome integrity. Centrosomes consist of a centriole pair surrounded by a protein network known as pericentriolar material (PCM). PCM assembly is a tightly regulated, critical step that determines the size and capability of centrosomes. Here, we report a role for tubulin in regulating PCM recruitment through the conserved centrosomal protein Sas-4. Tubulin directly binds to Sas-4; together they are components of cytoplasmic complexes of centrosomal proteins. A Sas-4 mutant, which cannot bind tubulin, enhances centrosomal protein complex formation and has abnormally large centrosomes with excessive activity. These results suggest that tubulin negatively regulates PCM recruitment. Whereas tubulin-GTP prevents Sas-4 from forming protein complexes, tubulin-GDP promotes it. Thus, the regulation of PCM recruitment by tubulin depends on its GTP/GDP-bound state. These results identify a role for tubulin in regulating PCM recruitment independent of its well-known role as a building block of microtubules. On the basis of its guanine-bound state, tubulin can act as a molecular switch in PCM recruitment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.