ArticleJournal of pharmaceutical and biomedical analysis2012
A novel ultrasensitive LC-MS/MS assay for quantification of intracellular raltegravir in human cell extracts.
Article in Journal of pharmaceutical and biomedical analysis, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Quantification of nine antiretroviral drugs in cerebrospinal fluid: An approach to overcome sample collection tube adsorption.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2023Article
- Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.Journal of acquired immune deficiency syndromes (1999) · 2020Article
- Cervicovaginal and Rectal Fluid as a Surrogate Marker of Antiretroviral Tissue Concentration: Implications for Clinical Trial Design.Journal of acquired immune deficiency syndromes (1999) · 2016Article
- Selective and rapid determination of raltegravir in human plasma by liquid chromatography-tandem mass spectrometry in the negative ionization mode.Journal of pharmaceutical analysis · 2015Article
- A rapid spin through oil results in higher cell-associated concentrations of antiretrovirals compared with conventional cell washing.Bioanalysis · 2015Article
- Eradicating HIV-1 infection: seeking to clear a persistent pathogen.Nature reviews. Microbiology · 2014Review
- Correlation between plasma, intracellular, and cervical tissue levels of raltegravir at steady-state dosing in healthy women.Antimicrobial agents and chemotherapy · 2014Article
- Persistent HIV-1 replication is associated with lower antiretroviral drug concentrations in lymphatic tissues.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Differential penetration of raltegravir throughout gastrointestinal tissue: implications for eradication and cure.AIDS (London, England) · 2013Article
- Antiretroviral bioanalysis methods of tissues and body biofluids.Bioanalysis · 2013Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
An assay using ultrahigh pressure liquid chromatography and mass spectrometry detection was developed and validated for measurement of the HIV integrase inhibitor raltegravir (MK-0518) in human cell extracts. The assay is designed to utilize 200 μl of 70% MeOH cell extract derived from human peripheral blood mononuclear cells or human tissue samples. The assay is linear over a range from 0.0023 to 9.2 ng ml(-1). The average %CV (SD/Mean)*100 and %deviation ((observed-target)/target)*100 were less than 20% at the lower limit of quantification and less than 15% over the range of the curve. This assay is an accurate and highly sensitive method for determining raltegravir concentrations in cellular extracts with a lower limit 40 to over 100-fold lower than other methods in the literature. We also present a new processing method where a rapid spin through oil produced a significant increase in apparent intracellular raltegravir concentration compared with conventional processing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.