ArticleLife sciences2012
Chronic endothelin-1 infusion elevates glomerular sieving coefficient and proximal tubular albumin reuptake in the rat.
Article in Life sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Sex-Specific Differences in Kidney Function and Blood Pressure Regulation.International journal of molecular sciences · 2024Review
- Mechanism of protective actions of sparsentan in the kidney: lessons from studies in models of chronic kidney disease.Clinical science (London, England : 1979) · 2024Review
- Albumin uptake and processing by the proximal tubule: physiological, pathological, and therapeutic implications.Physiological reviews · 2022Review
- Immune cell behaviour and dynamics in the kidney - insights from in vivo imaging.Nature reviews. Nephrology · 2022Review
- The Indiana O'Brien Center for Advanced Renal Microscopic Analysis.American journal of physiology. Renal physiology · 2021Review
- Tauroursodeoxycholic acid (TUDCA) abolishes chronic high salt-induced renal injury and inflammation.Acta physiologica (Oxford, England) · 2019Article
- Prevention of the progression of renal injury in diabetic rodent models with preexisting renal disease with chronic endothelin A receptor blockade.American journal of physiology. Renal physiology · 2018Article
- Association between Endothelin-1 Levels and Kidney Disease among Blacks.Journal of the American Society of Nephrology : JASN · 2017Article
- Surgical preparation of rats and mice for intravital microscopic imaging of abdominal organs.Methods (San Diego, Calif.) · 2017Article
- Dual inhibition of renin-angiotensin-aldosterone system and endothelin-1 in treatment of chronic kidney disease.American journal of physiology. Regulatory, integrative and comparative physiology · 2016Review
- Endothelin-1 and the kidney: new perspectives and recent findings.Current opinion in nephrology and hypertension · 2016Review
- Finding the bottom and using it: Offsets and sensitivity in the detection of low intensity values in vivo with 2-photon microscopy.Intravital · 2014Article
- Using 2-photon microscopy to understand albuminuria.Transactions of the American Clinical and Climatological Association · 2014Review
- Endothelin antagonists for diabetic and non-diabetic chronic kidney disease.British journal of clinical pharmacology · 2013Review
- Endothelin ET(B) receptors contribute to sex differences in blood pressure elevation in angiotensin II hypertensive rats on a high-salt diet.Clinical and experimental pharmacology & physiology · 2013Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
aimWe have previously found that chronic endothelin-1 (ET-1) infusion in Sprague-Dawley rats increases glomerular permeability to albumin (P(alb)) as assessed in vitro independent of blood pressure with no observed albuminuria. In this study, we hypothesized that ET-1 increases glomerular albumin filtration with accompanied increase in albumin uptake via the proximal tubule, which masks the expected increase in urinary albumin excretion. MAIN
methodsNonfasting Munich-Wistar Fromter rats were surgically prepared for in vivo imaging (n=6). Rats were placed on the microscope stage with the exposed kidney placed in a cover slip-bottomed dish bathed in warm isotonic saline. Rats were then injected i.v. with rat serum albumin conjugated to Texas Red that was observed to enter capillary loops of superficial glomeruli, move into Bowman's space, bind to the proximal tubular cell brush border and reabsorbed across the apical membrane. Glomerular sieving coefficient (GSC) was calculated as the ratio of conjugated albumin within the glomerular capillary versus that in Bowman's space. Rats were again studied after 2 weeks of chronic ET-1 (2 pmol/kg/min; i.v. osmotic minipump). KEY
findingsGlomerular sieving coefficient was significantly increased in rats following chronic ET-1 infusion (0.025 ± 0.005 vs. 0.017 ± 0.003, p<0.05). Mean fluorescence intensity for conjugated albumin within proximal tubules was increased by ET-1 infusion: 118.40 ± 6.34 vs. 74.27 ± 4.45 pixel intensity (p<0.01). SIGNIFICANCE: These data provide in vivo evidence that ET-1 directly increases glomerular permeability to albumin and that albuminuria is prevented by increased PT albumin uptake in the rat.
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