ArticleAnalytical chemistry2012
Enrichment and detection of rare proteins with aptamer-conjugated gold nanorods.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 79 citations in OpenAlex.
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- Gold nanocrystals: optical properties, fine-tuning of the shape, and biomedical applications.RSC advances · 2022Review
- A disposable gold foil paper-based aptasensor for detection of enteropathogenic Escherichia coli with SERS analysis and magnetic separation technology.Mikrochimica acta · 2021Article
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- Investigation of electrochemical behavior of potassium ferricyanide/ferrocyanide redox probes on screen printed carbon electrode through cyclic voltammetry and electrochemical impedance spectroscopy.Turkish journal of chemistry · 2021Article
- DNAzyme-functionalized porous carbon nanospheres serve as a fluorescent nanoprobe for imaging detection of microRNA-21 and zinc ion in living cells.Mikrochimica acta · 2020Article
- Electrokinetic Mixing for Improving the Kinetics of an HbA1c Immunoassay.Scientific reports · 2019Article
- Article
- A sensitive gold-nanorods-based nanobiosensor for specific detection of Campylobacter jejuni and Campylobacter coli.Journal of nanobiotechnology · 2019Article
- One-Step Facile Synthesis of Aptamer-Modified Graphene Oxide for Highly Specific Enrichment of Human A-Thrombin in Plasma.Sensors (Basel, Switzerland) · 2017Article
- Nucleic acid-functionalized transition metal nanosheets for biosensing applications.Biosensors & bioelectronics · 2017Review
- Fluorescence based Aptasensors for the determination of hepatitis B virus e antigen.Scientific reports · 2016Article
- BSA modification to reduce CTAB induced nonspecificity and cytotoxicity of aptamer-conjugated gold nanorods.Nanoscale · 2015Article
- A cell-targeted, size-photocontrollable, nuclear-uptake nanodrug delivery system for drug-resistant cancer therapy.Nano letters · 2015Article
- Functional DNA-containing nanomaterials: cellular applications in biosensing, imaging, and targeted therapy.Accounts of chemical research · 2014Article
- Post-genomics nanotechnology is gaining momentum: nanoproteomics and applications in life sciences.Omics : a journal of integrative biology · 2014Review
- Cancer cell sensing and therapy using affinity tag-conjugated gold nanorods.Interface focus · 2013Review
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Rare protein enrichment and sensitive detection hold great potential in biomedical studies and clinical practice. This work describes the use of aptamer-conjugated gold nanorods for the efficient enrichment of rare proteins from buffer solutions and human plasma. Gold nanorod (AuNR) surfaces were modified with a long PEG chain and a 15-mer thrombin aptamer for protein enrichment and detection. Studies of the effect of surface modification on enrichment efficiency of thrombin showed that a change of only one EG(6) linker unit, i.e., from 2EG(6) to 3EG(6), could increase thrombin protein capture efficiency by up to 47%. Furthermore, a 1 ppm sample of thrombin in buffer could be enriched with around 90% efficiency using a low concentration (0.19 nM) of gold nanorod probe modified with 3EG(6) spacer, and with the same probe, effective capture was achieved down to 10 ppb (1 ng) thrombin in plasma samples. In addition to α-thrombin enrichment, prothrombin was also efficiently captured from plasma samples via gold nanorods conjugated with 15-mer thrombin aptamer. Our work demonstrates efficient enrichment of rare proteins using aptamer-modified nanomaterials, which can be used in biomarker discovery studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.