ArticleJournal of proteome research2012
Metabolic characterization of Leishmania major infection in activated and nonactivated macrophages.
Article in Journal of proteome research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Review
- Article
- Metabolomics analysis of visceral leishmaniasis based on urine of golden hamsters.Parasites & vectors · 2023Article
- Gene Expression Profiling of Classically Activated Macrophages inTropical medicine and infectious disease · 2023Article
- A Nuclear Magnetic Resonance-Based Metabolomic Study to Identify Metabolite Differences between Iranian Isolates ofIranian journal of medical sciences · 2021Article
- Tissue/Biofluid Specific Molecular Cartography ofFrontiers in cellular and infection microbiology · 2021Article
- Mitochondrial Regulation of Macrophage Response Against Pathogens.Frontiers in immunology · 2020Review
- Metabolic programming of macrophage functions and pathogens control.Redox biology · 2019Review
- In Search of Biomarkers for Pathogenesis and Control of Leishmaniasis by Global Analyses ofFrontiers in cellular and infection microbiology · 2018Review
- Intracellular Survival of Leishmania major Depends on Uptake and Degradation of Extracellular Matrix Glycosaminoglycans by Macrophages.PLoS pathogens · 2015Article
- Proteomic-based approach to gain insight into reprogramming of THP-1 cells exposed to Leishmania donovani over an early temporal window.Infection and immunity · 2015Article
- Discovery of Infection Associated Metabolic Markers in Human African Trypanosomiasis.PLoS neglected tropical diseases · 2015Article
- Changes in metabolic phenotypes of Plasmodium falciparum in vitro cultures during gametocyte development.Malaria journal · 2014Article
- Metabolism via Arginase or Nitric Oxide Synthase: Two Competing Arginine Pathways in Macrophages.Frontiers in immunology · 2014Review
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Authors and funding
5 authors.
Funding
Abstract
Infection with Leishmania spp. can lead to a range of symptoms in the affected individual, depending on underlying immune-metabolic processes. The macrophage activation state hereby plays a key role. Whereas the l-arginine pathway has been described in detail as the main biochemical process responsible for either nitric oxide mediated parasite killing (classical activation) or amplification of parasite replication (alternative activation), we were interested in a wider characterization of metabolic events in vitro. We therefore assessed cell growth medium, parasite extract, and intra- and extracellular metabolome of activated and nonactivated macrophages, in presence and absence of Leishmania major. A metabolic profiling approach was applied combining 1H NMR spectroscopy with multi- and univariate data treatment. Metabolic changes were observed along both conditional axes, that is, infection state and macrophage activation, whereby significantly higher levels of potential parasite end products were found in parasite exposed samples including succinate, acetate, and alanine, compared to uninfected macrophages. The different macrophage activation states were mainly discriminated by varying glucose consumption. The presented profiling approach allowed us to obtain a metabolic snapshot of the individual biological compartments in the assessed macrophage culture experiments and represents a valuable read out system for further multiple compartment in vitro studies.
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