ArticleOpen biology2012
Transcriptome sequencing of black grouse (Tetrao tetrix) for immune gene discovery and microsatellite development.
Article in Open biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Review
- RNA sequencing of kidney and liver transcriptome obtained from wild cynomolgus macaque (Macaca fascicularis) originating from Peninsular Malaysia.BMC research notes · 2018Article
- Development of Microsatellite Markers for the Nipa Palm Hispid Beetle,The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2018Article
- Article
- Article
- De novo assembly of the dual transcriptomes of a polymorphic raptor species and its malarial parasite.BMC genomics · 2015Article
- How and why should we implement genomics into conservation?Evolutionary applications · 2014Review
- Article
- Whole genome sequencing of the black grouse (Tetrao tetrix): reference guided assembly suggests faster-Z and MHC evolution.BMC genomics · 2014Article
- Identification of differentially expressed genes in breast muscle and skin fat of postnatal Pekin duck.PloS one · 2014Article
- Combining next-generation sequencing and online databases for microsatellite development in non-model organisms.Scientific reports · 2013Article
- Brain transcriptome of the violet-eared waxbill Uraeginthus granatina and recent evolution in the songbird genome.Open biology · 2013Article
- Transcriptome of the dead: characterisation of immune genes and marker development from necropsy samples in a free-ranging marine mammal.BMC genomics · 2013Article
- Characterization of Liaoning cashmere goat transcriptome: sequencing, de novo assembly, functional annotation and comparative analysis.PloS one · 2013Article
- Sequencing of the core MHC region of black grouse (Tetrao tetrix) and comparative genomics of the galliform MHC.BMC genomics · 2012Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The black grouse (Tetrao tetrix) is a galliform bird species that is important for both ecological studies and conservation genetics. Here, we report the sequencing of the spleen transcriptome of black grouse using 454 GS FLX Titanium sequencing. We performed a large-scale gene discovery analysis with a focus on genes that might be related to fitness in this species and also identified a large set of microsatellites. In total, we obtained 182 179 quality-filtered sequencing reads that we assembled into 9035 contigs. Using these contigs and 15 794 length-filtered (greater than 200 bp) singletons, we identified 7762 transcripts that appear to be homologues of chicken genes. A specific BLAST search with an emphasis on immune genes found 308 homologous chicken genes that have immune function, including ten major histocompatibility complex-related genes located on chicken chromosome 16. We also identified 1300 expressed sequence tag microsatellites and were able to design suitable flanking primers for 526 of these. A preliminary test of the polymorphism of the microsatellites found 10 polymorphic microsatellites of the 102 tested. Genomic resources generated in this study should greatly benefit future ecological, evolutionary and conservation genetic studies on this species.
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