ArticlePLoS neglected tropical diseases2012
Oligonucleotide based magnetic bead capture of Onchocerca volvulus DNA for PCR pool screening of vector black flies.
Article in PLoS neglected tropical diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Evaluating the diagnostic test accuracy of molecular xenomonitoring methods for characterising the community burden of Onchocerciasis.PLoS neglected tropical diseases · 2021Pooled it
- Validation of a species-specific probe-based qPCR for detection ofInternational journal for parasitology. Parasites and wildlife · 2024Article
- Two Nigerian States (Plateau and Nasarawa) Have Eliminated Transmission of Human Onchocerciasis-A Report of Post-Ivermectin Mass Drug Administration Surveillance.The American journal of tropical medicine and hygiene · 2023Article
- The interruption of transmission of onchocerciasis in Kaduna, Kebbi and Zamfara states, Nigeria: another milestone achievement.International health · 2022Article
- Photo-Cross-Linked Probe-Modified Magnetic Particles for the Selective and Reliable Recovery of Nucleic Acids.ACS omega · 2022Article
- Article
- Historical Elimination of Onchocerciasis from Victoria Nile Focus in Central Uganda Verified Using WHO Criteria.The American journal of tropical medicine and hygiene · 2020Article
- The Interruption of Transmission of Human Onchocerciasis by an Annual Mass Drug Administration Program in Plateau and Nasarawa States, Nigeria.The American journal of tropical medicine and hygiene · 2020Article
- Post-elimination surveillance in formerly onchocerciasis endemic focus in Southern Mexico.PLoS neglected tropical diseases · 2020Article
- Comparison of PCR Methods forThe American journal of tropical medicine and hygiene · 2018Article
- Evaluation of Community-Directed Operation of Black Fly Traps for Entomological Surveillance ofThe American journal of tropical medicine and hygiene · 2017Article
- An isothermal DNA amplification method for detection of Onchocerca volvulus infection in skin biopsies.Parasites & vectors · 2016Article
- Stage-specific Proteomes from Onchocerca ochengi, Sister Species of the Human River Blindness Parasite, Uncover Adaptations to a Nodular Lifestyle.Molecular & cellular proteomics : MCP · 2016Article
- Detection of Onchocerca volvulus in Skin Snips by Microscopy and Real-Time Polymerase Chain Reaction: Implications for Monitoring and Evaluation Activities.The American journal of tropical medicine and hygiene · 2016Article
- Elimination of Onchocerciasis from Mexico.PLoS neglected tropical diseases · 2015Article
- Evaluation of a community-based trapping program to collect Simulium ochraceum sensu lato for verification of onchocerciasis elimination.PLoS neglected tropical diseases · 2014Article
- A simple isothermal DNA amplification method to screen black flies for Onchocerca volvulus infection.PloS one · 2014Article
- Detection of Onchocerca volvulus in Latin American black flies for pool screening PCR using high-throughput automated DNA isolation for transmission surveillance.Parasitology research · 2013Article
- Onchocerca volvulus-neurotransmitter tyramine is a biomarker for river blindness.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundEntomological surveys of Simulium vectors are an important component in the criteria used to determine if Onchocerca volvulus transmission has been interrupted and if focal elimination of the parasite has been achieved. However, because infection in the vector population is quite rare in areas where control has succeeded, large numbers of flies need to be examined to certify transmission interruption. Currently, this is accomplished through PCR pool screening of large numbers of flies. The efficiency of this process is limited by the size of the pools that may be screened, which is in turn determined by the constraints imposed by the biochemistry of the assay. The current method of DNA purification from pools of vector black flies relies upon silica adsorption. This method can be applied to screen pools containing a maximum of 50 individuals (from the Latin American vectors) or 100 individuals (from the African vectors). METHODOLOGY/PRINCIPAL
findingsWe have evaluated an alternative method of DNA purification for pool screening of black flies which relies upon oligonucleotide capture of Onchocerca volvulus genomic DNA from homogenates prepared from pools of Latin American and African vectors. The oligonucleotide capture assay was shown to reliably detect one O. volvulus infective larva in pools containing 200 African or Latin American flies, representing a two-four fold improvement over the conventional assay. The capture assay requires an equivalent amount of technical time to conduct as the conventional assay, resulting in a two-four fold reduction in labor costs per insect assayed and reduces reagent costs to $3.81 per pool of 200 flies, or less than $0.02 per insect assayed. CONCLUSIONS/SIGNIFICANCE: The oligonucleotide capture assay represents a substantial improvement in the procedure used to detect parasite prevalence in the vector population, a major metric employed in the process of certifying the elimination of onchocerciasis.
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