ArticlePloS one2012
A novel ENU-mutation in ankyrin-1 disrupts malaria parasite maturation in red blood cells of mice.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- De novo variations of ANK1 gene caused hereditary spherocytosis in two Chinese children by affecting pre-mRNA splicing.BMC pediatrics · 2023Article
- A novel ENU-induced Cpox mutation causes microcytic hypochromic anemia in mice.Experimental animals · 2022Article
- Forward Genetics in Apicomplexa Biology: The Host Side of the Story.Frontiers in cellular and infection microbiology · 2022Review
- [Clinical and genetic features of children with hereditary spherocytosis: an analysis of 4 cases].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2019Article
- Genetic conflicts with Plasmodium parasites and functional constraints shape the evolution of erythrocyte cytoskeletal proteins.Scientific reports · 2018Article
- Enterobacteria and host resistance to infection.Mammalian genome : official journal of the International Mammalian Genome Society · 2018Review
- Host genetics in malaria: lessons from mouse studies.Mammalian genome : official journal of the International Mammalian Genome Society · 2018Review
- Erythrocyte β spectrin can be genetically targeted to protect mice from malaria.Blood advances · 2017Article
- Ankyrin-1 Gene Exhibits Allelic Heterogeneity in Conferring Protection Against Malaria.G3 (Bethesda, Md.) · 2017Article
- A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice.Scientific reports · 2016Article
- The mechanics of malaria parasite invasion of the human erythrocyte - towards a reassessment of the host cell contribution.Cellular microbiology · 2016Review
- Genome-wide analysis of chemically induced mutations in mouse in phenotype-driven screens.BMC genomics · 2015Article
- The influence of host genetics on erythrocytes and malaria infection: is there therapeutic potential?Malaria journal · 2015Review
- Practical murine hematopathology: a comparative review and implications for research.Comparative medicine · 2015Review
- Mouse ENU Mutagenesis to Understand Immunity to Infection: Methods, Selected Examples, and Perspectives.Genes · 2014Review
- A flow cytometric assay to quantify invasion of red blood cells by rodent Plasmodium parasites in vivo.Malaria journal · 2014Article
- Interaction of Plasmodium falciparum knob-associated histidine-rich protein (KAHRP) with erythrocyte ankyrin R is required for its attachment to the erythrocyte membrane.Biochimica et biophysica acta · 2014Article
- A deep intronic mutation in the ankyrin-1 gene causes diminished protein expression resulting in hemolytic anemia in mice.G3 (Bethesda, Md.) · 2013Article
- Characterization of ENU-induced Mutations in Red Blood Cell Structural Proteins.Computational and structural biotechnology journal · 2013Article
- Suppression of hepcidin expression and iron overload mediate Salmonella susceptibility in ankyrin 1 ENU-induced mutant.PloS one · 2013Article
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9 authors.
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Abstract
The blood stage of the plasmodium parasite life cycle is responsible for the clinical symptoms of malaria. Epidemiological studies have identified coincidental malarial endemicity and multiple red blood cell (RBC) disorders. Many RBC disorders result from mutations in genes encoding cytoskeletal proteins and these are associated with increased protection against malarial infections. However the mechanisms underpinning these genetic, host responses remain obscure. We have performed an N-ethyl-N-nitrosourea (ENU) mutagenesis screen and have identified a novel dominant (haploinsufficient) mutation in the Ank-1 gene (Ank1(MRI23420)) of mice displaying hereditary spherocytosis (HS). Female mice, heterozygous for the Ank-1 mutation showed increased survival to infection by Plasmodium chabaudi adami DS with a concomitant 30% decrease in parasitemia compared to wild-type, isogenic mice (wt). A comparative in vivo red cell invasion and parasite growth assay showed a RBC-autonomous effect characterised by decreased proportion of infected heterozygous RBCs. Within approximately 6-8 hours post-invasion, TUNEL staining of intraerythrocytic parasites, showed a significant increase in dead parasites in heterozygotes. This was especially notable at the ring and trophozoite stages in the blood of infected heterozygous mutant mice compared to wt (p<0.05). We conclude that increased malaria resistance due to ankyrin-1 deficiency is caused by the intraerythrocytic death of P. chabaudi parasites.
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